南方农业学报2025，Vol.56Issue(2)：496-507,12.DOI:10.3969/j.issn.2095-1191.2025.02.014
月季黑斑病菌ISSR遗传多样性分析
ISSR genetic diversity analysis of rose black spot pathogen
摘要
Abstract
[Objective]This study aimed to investigate the species composition,genetic diversity and phylogenetic re-lationships of the rose black spot pathogen in China,which could provide theoretical basis for resistance identification of rose germplasm against black spot and disease control strategies.[Method]The pathogen of rose black spot were isolated from 27 cities(counties)of 15 provinces(autonomous regions)in 7 regions of China,and the pathogen species were de-termined by amplified strain ITS sequence,and the pathogenicity was verified by Koch's rule.The genetic diversity of the pathogens was studied using the ISSR molecular marker technology.The band data obtained by amplification was re-corded as a 0-1 matrix,and the genetic diversity index and genetic distance were calculated using PopGene 32.0,and the unweighted pair group method with arithmetic mean(UPGMA)clustering analysis was performed using NTsys-pc 2.1.[Result]A total of 107 strains of pathogen were isolated,which could be divided into 27 populations according to different cities(counties).All 107 strains were identified as Marssonina rosae.Three primers for ISSR-PCR amplification were identified:UBC884,UBC885 and UBC886.These primers produced a total of 84 bands,all of which were polymorphic,indicating a polymorphism rate of 100%,thus demonstrating high polymorphism,and it was suitable for the amplification of M.rosae.Genetic diversity analysis of 107 strains showed that the mean observed number of alleles(Na),mean effec-tive number of alleles(Ne),mean Nei's diversity index(H)and mean Shannon's information index(I)were 1.3369,1.2431,0.1367,0.1993,respectively.Average genetic diversity(Ht)was 0.2827,average genetic diversity within popula-tion(Hs)was 0.1368,average coefficient of genetic differentiation(Gst)was 0.5163,and gene flow(Nm)was 0.4684.Based on UPGMA cluster analysis,the 107 M.rosae strains could be divided into 8 groups when the genetic coefficient was 0.57.In groups Ⅰ-Ⅶ,strains from different regions were mixed with each other,while some strains in group VIII were grouped together based on their geographical location of cities(counties).[Conclusion]The 107 M.rosae strains from different geographical regions exhibit rich genetic diversity,low gene flow among populations,and high genetic dif-ferentiation.Moreover,there is no strong correlation between the genetic diversity of the strains and their geographical ori-gin.关键词
月季黑斑病/蔷薇盘二孢/ISSR分子标记/遗传多样性Key words
rose black spot/Marssonina rosae/ISSR molecular marker/genetic diversity分类
农业科技引用本文复制引用
郭加启,陈锋,吕博,杨园园,过聪,宋居荣,杨捷,向发云,林建国..月季黑斑病菌ISSR遗传多样性分析[J].南方农业学报,2025,56(2):496-507,12.基金项目
湖北省技术创新计划项目(2024BBB022) （2024BBB022）
武汉市知识创新专项(202302020101010141) （202302020101010141）
湖北省工业微生物重点实验室开放基金项目(202209KF10) （202209KF10）
特色花卉生物育种湖北省工程研究中心开放基金项目(2023ZD001) Hubei Technology Innovation Plan Project(2024BBB022) （2023ZD001）
Wuhan Knowledge Innovation Spe-cial Project(202302020101010141) （202302020101010141）
Open Fund Project of Hubei Key Laboratory of Industrial Microbiology(202209KF10) （202209KF10）
Open Fund Project of Hubei Engineering Research Center for Biological Breeding of Specialty Flowers(2023ZD001) （2023ZD001）
