南方农业学报2025，Vol.56Issue(2)：508-519,12.DOI:10.3969/j.issn.2095-1191.2025.02.015
大花海棠炭疽病病原菌鉴定、生物学特性测定及防治药剂室内毒力测定
Identification of the pathogen causing Begonia×benariensis an-thracnose,detection of the biological characteristics and indoor toxicity determination of control fungicides
摘要
Abstract
[Objective]To clarify the pathogenic species of Begonia×benariensis anthracnose and its biological chara-cteristics,to screen out the fungicides suitable for the control of this disease,which could lay the foundation for the re-search on the occurrence and development regulation of Begonia×benariensis anthracnose as well as the control measures.[Method]Pathogenic fungi were isolated from Begonia×benariensis plants showing typical anthracnose symptoms col-lected from the Kunming World Horticultural Expo Garden in Yunnan using the tissue isolation method.The pathogenicity of the isolated strains was confirmed according to Koch's law.The taxonomic identity of the anthracnose pathogen was de-termined by combining morphological observations with a multigene analysis of ITS,TUB2,HIS3,CHS-1,GAPDH and ACT.The biological characteristics of the pathogen were investigated by examining mycelial lethal temperature and myce-lial growth under different conditions,including various culture media,carbon sources,nitrogen sources,temperatures,illumination periods and pH levels.The inhibitory effects of 8 fungicides on the growth of the pathogen colony were as-sessed using mycelial growth rate method.[Result]Thirteen strains were isolated and purified from the pathogenic plants of Begonia×benariensis,and the thirteen strains were categorized as the same species according to colony morphology and microscopic morphology,and the representative strain SWBG5 was selected for the subsequent experiments.Accor-ding to morphological observations combined with multigene phylogenetic tree,the anthracnose pathogen SWBG5 was identified as Colletotrichum aotearoa.Biological experiments showed that PDA medium was beneficial for the growth of SWBG5 strain,with the fastest mycelial growth occurring when peptone was used as the nitrogen source,soluble starch as the carbon source,and at a temperature of 25℃.The mycelia of strain SWBG5 could grow well under 24 h light or 24 h darkness.The optimal pH was found to be 6,and the lethal temperature was 54℃.Indoor fungicide toxicity tests re-vealed that all the 8 fungicides inhibited the mycelial growth of strain SWBG5,with 30%pyraclostrobin SC exhibiting the strongest inhibitory effect,with an inhibitory concentration(EC50)value of 1.6959 μg/mL.[Conclusion]The patho-gen of anthracnose of Begonia×benariensis in the Kunming World Horticultural Expo Park in Yunnan is Colletotrichum aotearoa,and 30%pyrazoxystrobin can be recommended for controlling this disease in future treatment.关键词
大花海棠/炭疽病/病原菌/分离鉴定/生物学特性/毒力测定Key words
Begonia×benariensis/anthracnose/pathogen/isolation and identification/biological characteristics/toxicity test分类
农业科技引用本文复制引用
刘勉,肖玉辉,勾朝金,古旭,芦俊佳..大花海棠炭疽病病原菌鉴定、生物学特性测定及防治药剂室内毒力测定[J].南方农业学报,2025,56(2):508-519,12.基金项目
国家自然科学基金项目(32060695) （32060695）
云南省农业基础研究联合专项(202301BD070001-091) National Natural Science Foundation of China(32060695) （202301BD070001-091）
Yunnan Agricultural Fundamental Research Joint Special Project(202301BD070001-091) （202301BD070001-091）
