南方农业学报2025，Vol.56Issue(2)：520-532,13.DOI:10.3969/j.issn.2095-1191.2025.02.016
甘肃梨种质资源表型性状遗传多样性分析及优质资源筛选
Genetic diversity analysis of phenotypic traits of pear germplasm resources and fine germplasm resources screening in Gansu
摘要
Abstract
[Objective]This study aimed to analyze phenotypic traits genetic diversity of pear germplasm resources in Gansu,and screen fine germplasm resources which could provide theoretical basis for their evaluation and utilization of pear germplasm resources in Gansu.[Method]Fifty-three Gansu pear germplasm resources preserved were used as the re-search objects,and 29 phenotypic traits(17 quality traits and 12 quantitative traits)were observed.Genetic variation analysis,diversity analysis,correlation analysis,principal component analysis and cluster analysis were used to analyze the genetic diversity of Gansu pear germplasm resources.Finally,principal component analysis and membership function method were used to comprehensively evaluate the phenotypic traits of local pear germplasm resources in Gansu.[Result]The genetic diversity of 53 germplasm pear resources was rich,with the genetic variation coefficient of quantity traits ranging from 4.63%to 23.94%.The coefficient of variation was the highest for one-year-old branch length(23.94%),and was the lowest for stigma number(4.63%).The Simpson index(D)and Shannon-Wiener index(H´)of internode length were the highest in quantity traits,with values of 0.9857 and 4.099,D for bud color and Size of receptacle in quality traits were both the highest(0.9900),while H´ for number of lenticel was the highest(4.204).The correlation analysis results showed that except for the bud color,petal shape and leaf tip shape,which were not correlated with other traits,there was a certain correlation between the rest traits.Among them,there were 28 pairs and 25 pairs of traits that showed ex-tremely significant(P<0.01)and significant(P<0.05)correlations respectively.Principal component analysis revealed that the cumulative contribution rate of the first 5 principal components was 76.331%.Bud thickness and corolla diameter were main traits that affected phenotypic traits in pear.Comprehensive evaluation identified Wuwei Zhutoupear,Wudou Jitui,Gangu Mahuaxin pear as superior local germplasm resources.Cluster analysis showed that 53 pear germplasm re-sources were divided into 6 groups,and the phenotypic traits of each group were relatively consistent.Among them,groupⅠhad the largest one-year-old branch length and one-year-old branch thickness,group Ⅱ had a drooping tree pos-ture,and group Ⅵ had purple red anthers.[Conclusion]Gansu pear germplasm resources are rich in phenotypic diversity,germplasm genetic relationship is not necessarily related to its origin,so the local variety resources with fine comprehen-sive performance can be selected for pear germplasm innovation and new variety breeding.关键词
梨/表型性状/遗传多样性/综合评价/甘肃Key words
pear/phenotypic traits/genetic diversity/comprehensive evaluation/Gansu分类
农业科技引用本文复制引用
李晓斐,冯军仁,陈鹏,甄伟玲,郭爱,王玉凤,赖钟雄,陈斌..甘肃梨种质资源表型性状遗传多样性分析及优质资源筛选[J].南方农业学报,2025,56(2):520-532,13.基金项目
甘肃省中央引导地方科技发展资金项目(24ZYQG003) （24ZYQG003）
甘肃省林业和草原自列科技计划项目(2024kj101) （2024kj101）
张掖市科技计划项目(ZY2023BJ12,ZY2022KY05) Gansu Central Government Guiding Local Science and Technology Development Project(24ZYQG003) （ZY2023BJ12,ZY2022KY05）
Gansu Forestry and Grassland Self Listed Science and Technology Plan Project(2024kj101) （2024kj101）
Zhangye Science and Technology Plan Project(ZY2023BJ12,ZY2022KY05) （ZY2023BJ12,ZY2022KY05）
