南方农业学报2025，Vol.56Issue(2)：544-552,9.DOI:10.3969/j.issn.2095-1191.2025.02.018
澳洲坚果锌指蛋白基因MiZFP9的克隆与功能分析
Cloning and functional analysis of zinc finger protein gene MiZFP9 from macadamia
摘要
Abstract
[Objective]To clone CCCH type zinc finger protein gene MiZFP9 from macadamia(Macadamia integrifo-lia)and analyze its expression in different tissues and under cold and drought stresses,which could provide scientific ba-sis for molecular mechanism of zinc finger protein from macadamia in response to cold and drought stresses.[Method]Gene MiZFP9 was cloned from macadamia leaves,and bioinformatics analysis was conducted.The expression levels of MiZFP9 in different organs of macadamia and under cold and drought stresses were investigated by real-time fluorescence quantitative PCR technology.[Result]MiZFP9 gene was cloned from macadamia leaves and belonged to the C3H64 sub-class of CCCH type zinc finger protein gene.The encoded protein contained active sites,Zn2+binding sites as well as typi-cal domains ZF_C3H of CCCH type zinc finger protein,which was a stable hydrophilic protein and was mainly serine phosphorylation.It may be localized in the cytoplasm and was a non-secretory protein.The higher structure of MiZFP9 protein was mainly composed of random coil,α-helix and extended chain interlacing.MiZFP9 gene was expressed signifi-cantly differently in different tissues of Own Choice variety(P<0.05,the same below),among which the relative expres-sion level of MiZFP9 was the highest in newly opened and unopened flowers,followed by roots and leaves,while the relative expression level of MiZFP9 was the lowest in stems.Under low temperature stress and drought stress,the relative expression level of MiZFP9 gene in both Own Choice and HAES695 varieties was significantly up-regulated compared with the control(treated for 0 h),and the expression pattern of MiZFP9 gene was first increased and then decreased in Own Choice variety,the relative expression in HAES695 showed a fluctuating pattern with 2 peaks indicating that the re-sponse patters of different varieties were different.[Conclusion]MiZFP9 gene is specifically expressed in macadamia tis-sues,and is significantly up-regulated under cold and drought stresses,which speculates that MiZFP9 may play important positive regulatory roles in the growth and development of macadamia tissues and the response to cold and drought stresses.关键词
澳洲坚果/CCCH型锌指蛋白/基因克隆/功能分析Key words
macadamia/CCCH zinc finger protein/gene cloning/functional analysis分类
农业科技引用本文复制引用
杨祥燕,蔡元保,黄思婕,李季东,潘如军,黄运鹏..澳洲坚果锌指蛋白基因MiZFP9的克隆与功能分析[J].南方农业学报,2025,56(2):544-552,9.基金项目
国家自然科学基金项目(31860537) （31860537）
广西重点研发计划项目(桂农科AB241484005) （桂农科AB241484005）
广西农业科学院基本科研业务专项(桂农科2021YT154,桂农科2023ZX19) National Natural Science Foundation of China(31860537) （桂农科2021YT154,桂农科2023ZX19）
Guangxi Key Research and Develop-ment Plan Project(Guinongke AB241484005) （Guinongke AB241484005）
Basic Research Project of Guangxi Academy of Agricultural Sciences(Guinongke 2021YT154,Guinongke 2023ZX19) （Guinongke 2021YT154,Guinongke 2023ZX19）
