南方农业学报2025，Vol.56Issue(2)：553-562,10.DOI:10.3969/j.issn.2095-1191.2025.02.019
基于转录组测序的红肉火龙果淀粉降解相关基因挖掘及表达分析
Mining of starch-degrading related genes in red-fleshed dragon fruit and expression analysis based on transcriptome sequencing
摘要
Abstract
[Objective]To explore genes related to starch degradation in red-fleshed dragon fruit and analyze their ex-pression patterns at different developmental stages of the fruit,which could provide theoretical basis for elucidating the starch degradation pathway in the ripening process of red-fleshed dragon fruit.[Method]The tested red-fleshed dragon fruit variety was Ruanzhidahong.Four different developmental stages(19 d after flowering,22 d after flowering,25 d af-ter flowering and 28 d after flowering)were selected for transcriptomic sequencing,and the Fold Change≥2 and error discovery rate(FDR)<0.01 were used as the standard.Differentially expressed genes(DEGs)in fruits at different deve-lopmental stages were screened for GO functional annotation and KEGG signaling pathway enrichment analysis.Real-time fluorescence quantitative PCR was used to verify the reliability of transcriptome sequencing data and analyze the ex-pression patterns of candidate genes.[Result]A total of 22.84 Gb of clean data were obtained by transcriptome sequen-cing,with 76618317 clean reads,GC content of 44.78%-46.83%and Q20 ranging from 98.89%-99.74%,Q30 ranging from 96.73%to 98.53%.Compared with 19 d after flowering,there were 4194 DEGs on 28 d after flowering,of which 1728 were up-regulated and 2466 were down-regulated.The results of GO functional annotation analysis showed that the DEGs annotation in the comparison group at 28 d after flowering and 19 d after flowering were classified into 3 catego-ries:biological processes,cell components and molecular functions.In biological processes,the number of DEGs in-volved in cellular processes was the largest,followed by metabolic processes.The results of KEGG signaling pathway en-richment analysis showed that DEGs of the comparison group at 28 d after flowering and 19 d after flowering were signifi-cantly enriched in starch and sucrose metabolic pathways.Ten genes related to starch and sucrose metabolic pathways were screened,including glucan water dikinase(GWD)gene(HU02G00177),phosphoglucan water dikinase(PWD)gene(HU09G02021),phosphoglucan phosphatase(SEX)gene(HU07G01227),β-amylase(BAM)gene(HU10G00833,HU10G00777),α-amylase(AMY)gene(HU05G01323,HU02G00293,HU02G01323),isoamylase(ISA)gene(HU11G01417),and disproportionating enzyme(DPE)gene(HU09G01260).Real-time fluorescence quantitative PCR showed that the expression trend of 10 starch degrading genes was consistent with the transcriptome sequencing data.Rela-tive expression levels of HU02G00177,HU07G01227,HU10G00833,HU10G00777,HU02G00293 and HU11G01417 genes in the later stages of fruit development was higher than the early stage of fruit development.[Conclusion]The ex-pression patterns of genes related to starch degradation in red-fleshed dragon fruit are different at different developmental stages.Among them,GWD gene(HU02G00177),SEX gene(HU07G01227),BAM gene(HU10G00833,HU10G00777),AMY gene(HU02G00293),ISA gene(HU11G01417)may play an important role in the later stage of fruit development.关键词
红肉火龙果/淀粉降解/差异表达基因/转录组Key words
red-fleshed dragon fruit/starch degradation/differentially expressed genes/transcriptome分类
农业科技引用本文复制引用
魏治远,晏霜,解璞,郑乾明..基于转录组测序的红肉火龙果淀粉降解相关基因挖掘及表达分析[J].南方农业学报,2025,56(2):553-562,10.基金项目
国家自然科学基金项目(32060674) （32060674）
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2022]025-1) （黔科合平台人才-GCC[2022]025-1）
贵州省科技计划项目(黔科合中引地[2023]033) National Natural Science Foundation of China(32060674) （黔科合中引地[2023]033）
Guizhou High-level Innovative Ta-lent Project(QKHPTRC-GCC[2022]025-1) （QKHPTRC-GCC[2022]025-1）
Guizhou Science and Technology Plan Project(QKHZYD[2023]033) （QKHZYD[2023]033）
