南方农业学报2025，Vol.56Issue(2)：563-572,10.DOI:10.3969/j.issn.2095-1191.2025.02.020
甜瓜CmLEA基因家族鉴定及抗非生物胁迫基因挖掘
Identification of CmLEA gene family and mining of abiotic stress resistant genes in melon
摘要
Abstract
[Objective]To identify and analyze members of the late embryogenesis abundant(LEA)protein gene family in melon,mine the abiotic stress resistant genes,which could provide theoretical foundation for investigating the role of CmLEA gene family in abiotic stress responses and breeding of stress resistant melon varieties.[Method]Using cold tolerant melon L5283 seedlings as experimental materials.At the 3-leaf-and-1-bud stage,4 abiotic stress treatments were applied:salt stress[300 mmol/L sodium chloride(NaCl),drought[15%polyethylene glycol(PEG)6000],low temperature(15℃/6℃day/night,12 h/12 h),and abscisic acid(ABA)(100 μmol/L).Based on transcriptomic data from melon seedlings treated by low temperature,CmLEA candidate genes were identified.Chromosomal locations,gene structures,subcellular localization,secondary and tertiary protein structures,phylogenetic relationships,protein interactions,and promoter cis-acting elements were predicted by bioinformatics methods.Real-time fluorescence quantitative PCR was used to detect the expression patterns of CmLEA family genes under different abiotic stresses.[Result]A total of 5 CmLEA candidate genes were identified and named CmLEA1-CmLEA5.The 5 CmLEA family genes were unevenly distributed across 4 chromosomes of melon,they encoded 158-315 amino acids,with Theoretical isoelectric point(pI)of 4.47-9.70 and molecular weight of 16.90-32.71 kD.Except CmLEA2 were hydrophobic protein,the others were hydro-philic proteins.Among the 5 CmLEA family proteins,random curl accounted for the highest proportion,followed by extended chain and α-helix,and β-fold accounted for the lowest proportion.The conserved motifs of CmLEA family pro-teins differed greatly.CmLEA1 and CmLEA2 genes both had 1 exon and no intron;CmLEA3 and CmLEA5 genes con-tained 4 residues and 2 exons respectively.Phylogenetic analysis showed that CmLEA gene family members were closely related to cucumber,and the similarity was up to 100%.The cis-acting elements of CmLEA family genes were classified into 6 categories,including light response elements,hormone response elements,anaerobic induction elements,low tem-perature response elements,defense and stress response elements,and MYB binding elements.CmLEA5 protein played a key role in the protein-interaction network and was presumed to be involved in the development of stamens.Real-time fluorescence quantitative PCR showed that the relative expression of CmLEA1 and CmLEA2 were significantly up-regulated by low temperature and ABA induction(P<0.05,the same below).When melon seedlings were subjected to drought stress and salt stress,the relative expression of CmLEA5 gene reached its peak value at 12 h compared with 0 h,and significantly increased by 214 and 5212 times respectively.[Conclusion]Five CmLEA gene family members were identified,and their structure and function are diverse.Three genes,which are highly responsive to abiotic stress,are identified:CmLEA1,CmLEA2 and CmLEA5,and they can be used as candidate genes for subsequent studies on the mo-lecular mechanism of response to abiotic stress and selection of melon resistant varieties.关键词
甜瓜/LEA基因家族/非生物胁迫/表达分析Key words
melon/LEA gene family/abiotic stress/expression analysis分类
农业科技引用本文复制引用
杨帆,邹昌利,张利超,杜清洁,李娟起,汪虎,王吉庆,肖怀娟,李猛..甜瓜CmLEA基因家族鉴定及抗非生物胁迫基因挖掘[J].南方农业学报,2025,56(2):563-572,10.基金项目
国家自然科学基金项目(32202578) （32202578）
中国博士后科学基金项目(2022M711063) （2022M711063）
河南省大宗蔬菜产业技术体系岗位专家项目(HARS-22-07-G4) National Natural Science Foundation of China(32202578) （HARS-22-07-G4）
China Postdoctoral Science Founda-tion(2022M711063) （2022M711063）
Henan Bulk Vegetable Industry Technology System Post Expert Project(HARS-22-07-G4) （HARS-22-07-G4）
