南方农业学报2025，Vol.56Issue(2)：655-663,9.DOI:10.3969/j.issn.2095-1191.2025.02.029
高耐铅芽孢杆菌筛选及其对鸡毛菜吸收铅的阻控效应
Screening of Bacillus with high lead tolerance and its blocking effects on the lead uptake of Brassica chinensis
摘要
Abstract
[Objective]To screen microorganisms which had high tolerance to heavy metals,promoted plant growth and effectively blocked the transfer of heavy metals to the aboveground part of leafy vegetables,which could provide high-quality microbial resources for bioremediation of heavy metal contaminated soil.[Method]Bacillus was used as the test strain,which was screened for lead(Pb)resistant strains through selective media,and the maximum tolerance con-centration of Pb was determined using the dilution plating method.Different concentrations of Pb2+(25,50,100,200,400 mg/L)solutions were set up to analyze the strain's adsorption capacity for Pb using atomic absorption spectrometry.Germination experiments were conducted to analyze the growth promoting effects of the strain on the germination of Bras-sica chinensis seeds.Hydroponic experiments with different concentrations of Pb2+(0.3,30 mg/L)were conducted to in-vestigate the ability of the strain to block the absorption of Pb in the aboveground parts of Brassica chinensis.[Result]One strain of Bacillus subtilis was screened on LB solid medium with 1800 mg/L Pb2+concentration,which could grow normally and produce siderophore.Under conditions of natural pH,Pb2+initial concentration of 50 mg/L,wet strain con-centration of 16 g/L,temperature of 30℃,rotation speed of 150 r/min and adsorption time of 24 h,the strain achieved the highest Pb adsorption rate up to 96.1%.The seed germination experiment results showed that the Bacillus subtilis in-creased the germ length and root length of Brassica chinensis seeds under both Pb stress and non-stress conditions.The re-sults of the hydroponic experiment showed that,compared to non-inoculated treatments,at a higher Pb2+concentration(30 mg/L),the fresh weight and dry weight of B.chinensis treated with Bacillus subtilis increased significantly by 148.8%and 125.0%respectively(P<0.01),the inoculation treatments with bacterial strains at Pb2+concentrations of 0.3 and 30 mg/L significantly reduced the Pb content in the aboveground parts of Brassica chinensis by 53.5%and 38.9%(P<0.05,the same below)respectively,the Pb transfer coefficient was also significantly decreased by 23.1%and 20.5%respectively.[Conclusion]A strain of Bacillus subtilis has been screened,which not only exhibits high tolerance and adsorption capacity for Pb,but also shows significant plant growth promoting functions and the ability to block Pb trans-fer to the aboveground parts of Brassica chinensis.关键词
芽孢杆菌/鸡毛菜/重金属/铅/阻控作用Key words
Bacillus/Brassica chinensis/heavy metal/lead/block effect分类
农业科技引用本文复制引用
梁雪莲,陈伟,张璐,廖洁,蒋文艳,陆玮璠,王海军,王天顺..高耐铅芽孢杆菌筛选及其对鸡毛菜吸收铅的阻控效应[J].南方农业学报,2025,56(2):655-663,9.基金项目
广西自然科学基金面上项目(2024GXNSFAA010469) （2024GXNSFAA010469）
广西农业科学院基本科研业务专项(桂农科2021YT137,桂农科2023YM118) （桂农科2021YT137,桂农科2023YM118）
科技先锋队"强农富民""六个一"专项行动项目(桂农科盟202516) General Project of Guangxi Natural Science Foundation(2024GXNSFAA010469) （桂农科盟202516）
Basic Re-search Project of Guangxi Academy of Agricultural Sciences(Guinongke 2021YT137,Guinongke 2023YM118) （Guinongke 2021YT137,Guinongke 2023YM118）
Pioneer of Science and Technology's"Strengthening Agriculture and Enriching the People"and"Six Ones"Special Actions(Gui-nongkemeng 202516) （Gui-nongkemeng 202516）
