湖南中医药大学学报2025，Vol.45Issue(3)：395-401,7.DOI:10.3969/j.issn.1674-070X.2025.03.001
薏苡仁汤对寒湿痹阻型胶原性关节炎大鼠Treg/Th17细胞失衡的调节作用
Regulatory effects of Yiyiren Decoction on Treg/Th17 cell imbalance in rats with collagen-induced arthritis of cold-dampness obstruction pattern
摘要
Abstract
Objective To observe the preventive and therapeutic effects of Yiyiren Decoction(YYRD)on collagen-induced arthritis(CIA)rats with cold-dampness obstruction pattern,and to investigate its impact on regulatory T cells(Treg)and T helper cell 17(Th17)cells.Methods A rat model was established by injecting type Ⅱ collagen combined with exposure to a climate chamber simulating a cold-dampness environment.Rats were randomly divided into blank group,model group,positive control group(leflunomide 10 mg/kg),and low-and high-dose YYRD groups(12 g/kg and 24 g/kg raw medicines,respectively),with eight rats in each group.The overall situation signs of the rats were observed daily,and the joint swelling in the right hind paw was measured weekly.After four weeks of administration,the histopathological changes in ankle joint sections were observed under an optical microscope.ELISA was used to determine the levels of rheumatoid factor(RF),anti-cyclic citrullinated peptide antibody(ACPA),C-reactive protein(CRP),serum transforming growth factor-β(TGF-β),and interleukin-17A(IL-17A),which are indicators of disease activity.Flow cytometry was used to check the proportions of CD4+CD25+Foxp3+Treg cells and CD4+IL-17+Th17 cells among CD4+T cells in peripheral blood.Results The overall situation signs of the rats in the model group were consistent with CIA of cold-dampness obstruction pattern,with visible joint stiffness,deformity,and limited mobility.The overall situation and joint symptoms of the rats in the low-and high-dose YYRD groups were alleviated,and the degree of joint swelling was lower than that in the model group at three and four weeks after administration(P<0.01).After four weeks of administration,compared with the model group,both the low-and high-dose YYRD groups showed varying degrees of reduction in ankle joint inflammatory cell infiltration,synovial/fibrous tissue hyperplasia,and bone and cartilage structure damage.The serum levels of RF,ACPA,and CRP were all reduced(P<0.05,P<0.01),the proportion of Treg cells in peripheral blood and the level of their signature cytokine TGF-β increased(P<0.01),and the proportion of Th17 cells and the level of their signature cytokine IL-17A decreased(P<0.01).The serum level of TGF-β in the high-dose YYRD group was higher than that in the low-dose YYRD group(P<0.05),with no statistically significant differences in other indicators between the two groups(P>0.05).Conclusion YYRD can relieve the overall situation signs and joint symptoms of rats with CIA of cold-dampness obstruction pattern.Its mechanism of action may be related to regulating the immune imbalance between Treg/Th17 cells,reducing inflammatory reactions in joint tissue and the whole body,and alleviating bone and cartilage damage.关键词
胶原性关节炎/寒湿痹阻证/薏苡仁汤/调节性T细胞/辅助性T细胞17/转化生长因子-β/白细胞介素-17AKey words
collagen-induced arthritis/cold-dampness obstruction pattern/Yiyiren Decoction/regulatory T cells/T helper cell 17/transforming growth factor-β/interleukin-17A分类
医药卫生引用本文复制引用
刘鑫鑫,田松云,崔建博,葛炎,张国斌..薏苡仁汤对寒湿痹阻型胶原性关节炎大鼠Treg/Th17细胞失衡的调节作用[J].湖南中医药大学学报,2025,45(3):395-401,7.基金项目
河南省高校科技创新人才支持计划项目(15HASTIT041). （15HASTIT041）
