湖南中医药大学学报2025，Vol.45Issue(3)：402-408,7.DOI:10.3969/j.issn.1674-070X.2025.03.002
秦皮苷调节SDF-1/CXCR4信号通路对类风湿关节炎大鼠炎性反应的影响
Effects of fraxin on inflammatory response in rats with rheumatoid arthritis by regulating SDF-1/CXCR4 signaling pathway
摘要
Abstract
Objective To investigate the effects of fraxin on inflammatory response in rats with rheumatoid arthritis(RA)by regulating stromal cell-derived factor-1(SDF-1)/CXC chemokine receptor 4(CXCR4)signaling pathway.Methods Rats were randomly divided into normal group,RA group,low-dose(0.2 g/kg)fraxin group,high-dose(0.4 g/kg)fraxin group,high-dose(0.4 g/kg)fraxin+NUCC-390(2.2 mg/kg)group,with 10 rats in each group.The RA model was established with type II collagen induction in all groups except the normal group.After the intervention,the arthritis index(AI)and paw volume of rats in each group were measured.Micro-CT scanning was performed to assess the bone morphology of the ankle joints.Histopathological morphology of the ankle joint tissue was observed using HE staining.ELISA was used to test the levels of inflammatory cytokines interleukin(IL)-6,IL-18,tumor necrosis factor-α(TNF-α),C-reactive protein(CRP),and rheumatoid factor(RF)in the serum and synovial fluid.Western blot was performed to measure the protein expression levels of SDF-1 and CXCR4 in the ankle joint tissue.Results Compared with the normal group,the RA group exhibited increased levels of AI,paw volume,bone destruction score,histopathological morphological score of the ankle joint,and the levels of IL-6,IL-18,TNF-α,CRP,RF,as well as the protein expression levels of SDF-1 and CXCR4(P<0.05).Compared with the RA group,both the low-dose and high-dose fraxin groups showed decreased levels of AI,paw volume,bone destruction score,histopathological morphological score of the ankle joint,and the levels of IL-6,IL-18,TNF-α,CRP,RF,as well as the protein expression levels of SDF-1 and CXCR4(P<0.05).Furthermore,the high-dose fraxin group exhibited lower levels of these indicators compared to the low-dose fraxin group(P<0.05).Compared with the high-dose fraxin group,the high-dose fraxin+NUCC-390 group showed increased levels of AI,paw volume,bone destruction score,histopathological morphological score of the ankle joint,and the levels of IL-6,IL-18,TNF-α,CRP,RF,as well as the protein expression levels of SDF-1 and CXCR4(P<0.05).Conclusion Fraxin can alleviate the inflammatory response and relieve arthritis symptoms in RA rats by downregulating the SDF-1/CXCR4 signaling pathway.关键词
类风湿关节炎/秦皮苷/基质细胞衍生因子-1/CXC趋化因子受体4/炎性反应Key words
rheumatoid arthritis/fraxin/stromal cell-derived factor-1/CXC chemokine receptor 4/inflammatory response分类
医药卫生引用本文复制引用
韩天然,纪青灼,王小玉,吴琳,邰立仕,李泽光..秦皮苷调节SDF-1/CXCR4信号通路对类风湿关节炎大鼠炎性反应的影响[J].湖南中医药大学学报,2025,45(3):402-408,7.基金项目
黑龙江省自然科学基金项目(2023085J08). （2023085J08）
