湖南中医药大学学报2025，Vol.45Issue(3)：432-437,6.DOI:10.3969/j.issn.1674-070X.2025.03.006
降脂理肝汤抑制铁死亡改善非酒精性脂肪性肝病小鼠的研究
Study on Jiangzhi Ligan Decoction inhibiting ferroptosis and alleviating non-alcoholic fatty liver disease in mice
摘要
Abstract
Objective To investigate the effects of Jiangzhi Ligan Decoction(JZLGD)on non-alcoholic fatty liver disease(NAFLD)mice and explore its mechanism of action.Methods Sixty SPF-grade C57BL/6 mice were randomly divided into blank group,model group,ferroptosis inhibitor group(1 mg/kg-1),and high-,medium-,and low-dose JZLGD groups(13.30,6.60,and 3.30 g/kg-1).The NAFLD model was established by feeding the mice high fat diet(HFD)for 12 weeks.After successful modelling,each group was given the corresponding medication daily for six consecutive weeks.In this experiment,the serum levels of free fatty acids(FFA),triglyceride(TG),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)were determined in mice,the hepatic lipid deposition and pathological damage in liver tissue were examined by Oil Red O staining and HE staining,the mRNA expressions of Nrf2,SLC7A11,and GPX4 in liver tissue were checked by RT-qPCR,and the protein expressions of Nrf2,SLC7A11,and GPX4 in liver tissue were determined by Western blot.Results Compared with mice in the blank group,the mice in the model group exhibited increased liver weight and liver index(P<0.05),elevated liver function indicators ALT and AST(P<0.01,P<0.05),obvious steatosis in the liver,disordered hepatic lobule structure,and significantly reduced protein and mRNA expressions of Nrf2,SLC7A11,and GPX4(P<0.01).Compared with the model group,mice in the ferroptosis inhibitor group showed decreased liver weight and liver index(P<0.05,P<0.01),clear liver lobule structure,reduced serum levels of ALT,AST,and TG(P<0.01,P<0.05),and increased protein and mRNA expressions of Nrf2,SLC7A11,and GPX4(P<0.01).Mice in the high-dose JZLGD group showed decreased liver weight and liver index(P<0.05),significantly improved liver function,reduced blood lipids(TG and FFA)(P<0.05,P<0.01),and upregulated protein and mRNA expressions of Nrf2,SLC7A11,and GPX4(P<0.05).Mice in the medium-and low-dose JZLGD groups also showed decreased liver index(P<0.05,P<0.01),improved liver function,and reduced serum levels of FFA and TG(P<0.05,P<0.01).Conclusion JZLGD can reduce blood lipids,improve liver function,and alleviate NAFLD,potentially through inhibiting ferroptosis.关键词
非酒精性脂肪性肝病/铁死亡/降脂理肝汤/Nrf2/SLC7A11/GPX4Key words
non-alcoholic fatty liver disease/ferroptosis/Jiangzhi Ligan Decoction/Nrf2/SLC7A11/GPX4分类
医药卫生引用本文复制引用
薛小嫣,黎鹏程,尹抗抗..降脂理肝汤抑制铁死亡改善非酒精性脂肪性肝病小鼠的研究[J].湖南中医药大学学报,2025,45(3):432-437,6.基金项目
湖南省自然科学基金面上项目(2022JJ30446) （2022JJ30446）
湖南省教育厅优秀青年基金项目(21B0393). （21B0393）
