湖南中医药大学学报2025，Vol.45Issue(3)：438-444,7.DOI:10.3969/j.issn.1674-070X.2025.03.007
丹参通络解毒汤调控METTL3介导的m6A修饰对缺氧/复氧CMECs的保护作用
Protective effects of Danshen Tongluo Jiedu Decoction on hypoxia/reoxygenation-injured CMECs through regulating METTL3-mediated m6A modification
摘要
Abstract
Objective To explore the protective effects of Danshen Tongluo Jiedu Decoction(DSTLJDD)on cardiac microvascular endothelial cells(CMECs)of rats with hypoxia/reoxygenation(H/R)injury and its influence on methyltransferase-like 3(METTL3).Methods The H/R-injured CMECs model was established,and the cells were divided into blank group,model group,Chinese medicine(CM)group,overexpression group,CM+overexpression group,and empty vector group.The levels of inflammatory factors IL-1β and IL-10 in each group were determined by ELISA,cell apoptosis was measured by flow cytometry,the protein expression level of METTL3 was checked by Western blot,and the content of m6A and creatine kinase(CK)were examined by colorimetry.Results Compared with the blank group,the protein expression of METTL3 and the level of m6A in the model group increased(P<0.01),the apoptosis rate of CMECs increased(P<0.01),the content of IL-1β and CK increased(P<0.01),while the content of IL-10 decreased(P<0.01).Compared with the model group,the protein expression of METTL3 and the level of m6A in the CM group decreased(P<0.05,P<0.01),the apoptosis rate of CMECs markedly decreased(P<0.01),the content of IL-1β and CK significantly decreased(P<0.01),while the content of IL-10 notably increased(P<0.01).Compared with the model group,the protein expression of METTL3 in the overexpression group increased(P<0.01),the apoptosis rate of CMECs increased(P<0.01),the content of IL-1β and CK increased(P<0.01,P<0.05),while the content of IL-10 decreased(P<0.01).Compared with the overexpression group,the protein expression of METTL3 in the CM+overexpression group decreased(P<0.01),the apoptosis rate of CMECs decreased(P<0.01),the content of IL-1β and CK decreased(P<0.01,P<0.05),while the content of IL-10 significantly increased(P<0.01).Conclusion DSTLJDD can reduce inflammatory responses,inhibit cell apoptosis,and exert protective effects on H/R-injured CMECs.Its mechanism may be related to the suppression of METTL3 expression,thereby downregulating m6A level.关键词
丹参通络解毒汤/甲基转移酶样3/m6A修饰/心肌缺血再灌注损伤/心肌微血管内皮细胞Key words
Danshen Tongluo Jiedu Decoction/methyltransferase-like 3/m6A modification/myocardial ischemia-reperfu-sion injury/cardiac microvascular endothelial cells分类
医药卫生引用本文复制引用
陆哲雯,刘莉,蒋啸,李鑫辉..丹参通络解毒汤调控METTL3介导的m6A修饰对缺氧/复氧CMECs的保护作用[J].湖南中医药大学学报,2025,45(3):438-444,7.基金项目
国家自然科学基金面上项目(82074392) （82074392）
湖南省自然科学基金区域联合基金项目(2023JJ50036) （2023JJ50036）
湖南省中医药管理局重点项目(A2023013) （A2023013）
湖南省教育厅科学研究项目(23A0283). （23A0283）
