湖南中医药大学学报2025，Vol.45Issue(3)：453-459,7.DOI:10.3969/j.issn.1674-070X.2025.03.009
真武汤与芍药苷在系统性红斑狼疮中的作用及机制探讨
Effects and mechanisms of Zhenwu Decoction and paeoniflorin in treating systemic lupus erythematosus
摘要
Abstract
Objective To elucidate the therapeutic effects and mechanisms of Zhenwu Decoction(ZWD)and its key component paeoniflorin(PF)in systemic lupus erythematosus(SLE)based on network pharmacology and experimental studies.Methods The target gene information corresponding to PF,the key component of ZWD,was obtained from the TCMSP database.SLE-related target genes were retrieved from the GeneCards and OMIM platforms.Key targets were identified through Venn diagram analysis of the intersection between PF target genes and SLE disease target genes.The STRING platform was used to construct a protein-protein interaction network model for the key targets.The gene annotation and analysis platform DAVID 6.8 was employed for GO functional enrichment analysis and KEGG pathway enrichment analysis of the key targets.In vitro experiments validation,mouse macrophage-like cell line RAW 264.7 was selected.The experiment included blank control group,R848-induced group(RAW 264.7 cells were induced to inflammatory polarization with 8 μg/mL R848 for 48 h),PF treatment group(treated with 80 μmol/L PF for 24 h starting from the 24th hour of R848 induction),and 2.5%and 5%ZWD treatment groups(treated with 2.5%and 5%ZWD medicated serum for 24 h starting from the 24th hour of R848 induction).Flow cytometry was used to check the CD86 and CD206 expressions to determine the proportion of M1 and M2 macrophages and assess the effects of ZWD and PF on macrophage inflammatory polarization.Results Network pharmacology analysis revealed that there were 74 drug target genes in PF,and 26 of which were key pathogenic genes for SLE.GO functional enrichment analysis showed that PF mainly acted on cell chemotaxis,proliferation regulation,and enzyme binding processes.KEGG pathway enrichment analysis indicated that PF can regulate biological functions of monocytes/macrophages and inflammation-related pathways,especially the phosphatidylinositol 3-kinase(PI3K)/serine/threonine protein kinase(Akt)pathway,nuclear transcription factor(NF-κB)pathway,mitogen-activated protein kinase(MAPK)pathway,and Janus kinase/signal transducer and activator of transcription(JAK/STAT)pathway,which played core roles in the pathogenesis of SLE.In vitro cellular experiments showed that compared with the blank control group,the proportion of CD86 M1 macrophages in the R848-induced group significantly increased(P<0.001,P<0.000 1).Compared with the R848-induced group,the proportions of CD86 M1 macrophages in the PF group,2.5%ZWD group,and 5%ZWD group all notably decreased(P<0.001,P<0.000 1).Conclusion Chinese medicine compound formula ZWD and its key active component PF may exert their potential utility in the treatment of SLE by inhibiting the polarization of monocytes/macrophages towards the M1 phenotype and intervening in the inflammatory response.关键词
系统性红斑狼疮/真武汤/芍药苷/单核细胞/网络药理学Key words
systemic lupus erythematosus/Zhenwu Decoction/paeoniflorin/monocytes/network pharmacology分类
医药卫生引用本文复制引用
黄婷,朱权,陈纯静,佘颜,罗卉,卢芳国..真武汤与芍药苷在系统性红斑狼疮中的作用及机制探讨[J].湖南中医药大学学报,2025,45(3):453-459,7.基金项目
国家自然科学基金项目(82074250) （82074250）
湖南省自然科学基金青年项目(2025JJ60529) （2025JJ60529）
湖南省教育厅优秀青年项目(24B0341). （24B0341）
