湖南农业科学Issue(2)：13-16,4.DOI:10.16498/j.cnki.hnnykx.2025.002.003
竹柏腋芽离体培养再生芽诱导体系中激素配比优化研究
Optimization of Hormone Ratios in the Regenerated Shoot Induction System for in vitro Culture of Nageia nagi Axillary Buds
摘要
Abstract
Nageia nagi axillary buds were cultured in vitro in the basic media of MS supplemented with different mass ratios of NAA and 6-BA.The effects of different treatments on callus formation and regenerated shoot induction were observed to identify the optimal hormone ratio for callus and regenerated shoot induction systems.The results showed that both NAA and 6-BA had highly significant impacts on callus formation and shoot differentiation from stem-attached axillary buds of N.nagi and they exerted evident interactive effects.NAA had stronger effect on callus induction than 6-BA,and the optimal hormone combination was 0.50 mg/L 6-BA+0.05 mg/L NAA.For shoot differentiation from callus,6-BA had stronger effect than NAA,and the optimal hormone combination was 0.50 mg/L 6-BA+0.10 mg/L NAA.关键词
竹柏/腋芽离体培养/愈伤组织/再生芽Key words
Nageia nagi/in vitro culture of axillary buds/callus/regenerated shoots分类
农业科技引用本文复制引用
巩媛,吴超男,刘芳,陈建荣..竹柏腋芽离体培养再生芽诱导体系中激素配比优化研究[J].湖南农业科学,2025,(2):13-16,4.基金项目
长沙市科技计划重点项目(K1205040-31) （K1205040-31）
