吉林中医药2025，Vol.45Issue(3)：343-347,5.DOI:10.13463/j.cnki.jlzyy.2025.03.021
基于自噬探讨活血化痰饮治疗激素性股骨头坏死作用机制
Exploring the mechanism of Huoxue Huatan Yin on treating steroid-induced avascular necrosis of the femoral head based on autophagy
摘要
Abstract
Objective To explore the effects and mechanism of Huoxue Huatan Yin on steroid-induced avascular necrosis of the femoral head(SANFH)based on autophagy.Methods Thirty male New Zealand white rabbits were randomly divided into a blank control group(n=6)and a modeling group(n=24).A rabbit model of SANFH was established using Luo Tianyou's improved hormone combined with lipopolysaccharide.After the successful modeling was detected by MRI,the experimental rabbits were randomly divided into a model group,and low-,medium-,and high-dose traditional Chinese medicine groups.The blank control group and model group were given normal saline by gavage,while the low-,medium-,and high-dose traditional Chinese medicine groups were given different doses of traditional Chinese medicine by gavage,all for 8 consecutive weeks.The general conditions of the mental state,water and diet of the experimental rabbits were observed in each group.After the intervention,bilateral femoral heads were surgically removed,and their histological morphology and empty bone pit rates were compared through HE staining and light microscopy.Western blot was used to detect the protein expression of autophagy related proteins LC3II and Beclin-1.Relevant data were recorded and statistically analyzed using the SPSS 25.0 software.Results HE staining results showed that the blank control group had an intact bone trabecular structure,abundant bone marrow content,and a large number of hematopoietic cells visible in the medullary cavity,while the structure of bone trabeculae in the model group was disordered,with some bone trabeculae thinning or even broken,and a significant increase in adipocytes,and each traditional Chinese medicine group showed varying degrees of improvement in bone trabecular injury compared to the model group,with more abundant hematopoietic cells and intramedullary tissue.The results showed that the blank control group had only a small amount of empty bone pits in the femoral head tissue,and the rates of empty bone pits were higher in the model and traditional Chinese medicine groups than those in the blank control group(P<0.05).The empty bone pit rate of each traditional Chinese medicine group decreased significantly compared to that in the model group(P<0.05).Among them,the high-dose group showed the most significant decrease.Western blot results showed that the expression of autophagy related proteins LC3II and Beclin-1 was significantly enhanced after modeling,and the expression of autophagy related proteins in the model group and traditional Chinese medicine group was significantly increased compared to that in the blank control group(P<0.05),while the protein expression in the medium-and high-dose traditional Chinese medicine groups was significantly decreased compared to that in the model group(P<0.05).The protein expression in the low-dose group showed no significant difference compared to that in the model group.Conclusion Huoxue Huatan Yin can improve the expression of bone necrosis in SANFH model rabbits,and its mechanism maybe attributes to inhibiting the over-expression of autophagy to treat the disease.关键词
活血化痰饮/激素性股骨头坏死/自噬Key words
Huoxue Huatan Yin/steroid-induced avascular necrosis of the femoral head(SANFH)/autophagy分类
医药卫生引用本文复制引用
陈彦华,谢文鹏,牟淑敏,武凯,张宏建,王凤铭..基于自噬探讨活血化痰饮治疗激素性股骨头坏死作用机制[J].吉林中医药,2025,45(3):343-347,5.基金项目
山东省中医药科技发展计划项目(2019-0113) （2019-0113）
