江苏大学学报（医学版）2025，Vol.35Issue(2)：93-101,9.DOI:10.13312/j.issn.1671-7783.y240028
LncRNA SNHG11通过上调LIN28A表达促进胰腺癌细胞干性
LncRNA SNHG11 promotes stemness via up-regulating the expression of LIN28A in pancreatic cancer cells
摘要
Abstract
Objective:To explore the effect of long non-coding RNA(lncRNA)small nucleolar RNA host gene 11(SNHG11)on the proliferation and stemness of pancreatic cancer cells and its possible mechanisms.Methods:The expression of lncRNA SNHG11 in pan cancer and corresponding paracancerous tissues,pancreatic cancer and pancreatic tissues were analyzed by TCGA and GTEx databases;the expression of lncRNA SNHG11 in pancreatic cancer PANC1,PaTu8988 and MIApaca-2 cells was detected by qRT-PCR;pcDNA3.1 and pcDNA3.1-SNHG11 plasmids were transfected into PANC1 and PaTu8988 cells,respectively;sh-EGFP and sh-SNHG11 plasmids were transfected into PANC1 and MIApaca-2 cells,respectively.Plasmid transfection efficiency was measured by qRT-PCR,cell proliferation rate was measured by CCK-8 assay,clone formation experiment was used to detect the number and size of cell clones,qRT-PCR and Western blotting were used to detect the mRNA and protein levels of stemness indicators,including NANOG homeobox protein(NANOG),sex determining region of Y-chromosome related HMG-box2(SOX2),octamer binding transcription factor 4(OCT4),cellular oncogene myc(c-myc)and LIN28 homolog A(LIN28A),respectively.Stem cell spheroidization experiment was used to detect changes in the number and diameter of stem cell spheroidization;RNA immunoprecipitation assay was used to detect the binding of SNHG11 and LIN28A.Co-transfection of pcDNA3.1-SNHG11+sh-LIN28A or co-transfection of sh-SNHG11+3×Flag-LIN28A,qRT-PCR was used to detect the mRNA levels of stemness indicators NANOG,SOX2,OCT4,and c-myc.Stem cell spheroidization experiments were used to detect changes in the number and diameter of stem cell spheroidization.Results:SNHG11 is highly expressed in a variety of malignant tumors,including pancreatic cancer.The relative expression of lncRNA SNHG11 in pancreatic cancer cells from low to high was PaTu8988,PANC1,MIApaca-2(P<0.05).Up-regulating of SNHG11 expression significantly increased the proliferation and stemness of pancreatic cancer cells,while down-regulating exerted the opposite outcomes(P<0.05).LncRNA SNHG11 could bind to LIN28A.Overexpression of SNHG11 significantly increased the stemness of pancreatic cancer cells,and interference with LIN28A greatly reduced the stemness of pancreatic cancer cells,down-regulating LIN28A in pancreatic cancer cells with over-expression of SNHG11 markedly inhibited the stemness promotion of pancreatic cancer cells(P<0.05).By silencing SNHG11,the stemness ability of pancreatic cancer cells was significantly reduced,while overexpression of LIN28A significantly enhanced the stemness ability of pancreatic cancer cells.Upregulating the expression of LIN28A in pancreatic cancer cells with low expression of SNHG11 could significantly reverse the stemness inhibition of pancreatic cancer cells(P<0.05).Conclusion:LncRNA SNHG11 may promote the proliferation and enhance the stemness of pancreatic cancer cells by up-regulating the expression of LIN28A.关键词
长链非编码RNA(lncRNA)/核仁小分子RNA宿主基因 11(SNHG11)/LIN28A/干性/胰腺癌Key words
long non-coding RNA(lncRNA)/small nucleolar RNA host gene 11(SNHG11)/LIN28A/stemness/pancreatic cancer分类
临床医学引用本文复制引用
于正悦,王慧之,丁运韬,周雨静,龚爱华,徐岷..LncRNA SNHG11通过上调LIN28A表达促进胰腺癌细胞干性[J].江苏大学学报（医学版）,2025,35(2):93-101,9.基金项目
国家自然科学基金资助项目(82072754) （82072754）
江苏省卫生健康委员会面上项目(M2020011) （M2020011）
江苏省重点研发计划社会发展项目(BE2018689) （BE2018689）
镇江市重点研发计划社会发展项目(SH2018033) （SH2018033）
