LncRNA DNAH17-AS1在胃癌中的表达及意义OA

Objective:To investigate the expression levels and clinical significance of long non-coding RNA(lncRNA)dynein axonemal heavy chain 17-antisense strand 1(DNAH17-AS1)in gastric cancer tissues,as well as its effect on the biological function of gastric cancer cells and its possible mechanisms.Methods:Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the expression levels of lncRNA DNAH17-AS1 in tissue samples(108 gastric cancer tissues and corresponding adjacent tissues)and plasma samples(25 gastric cancer patients and 25 healthy people,respectively).Statistical analysis was carried out to analyze the correlation between the expression of DNAH17-AS1 and clinicopathological features of patients with gastric cancer.Gastric cancer AGS and HGC-27 cells were divided into si-NC control group and siRNA knockout group,respectively,and transfected with si-NC and si-DNAH17-AS1 by liposome transfection method.The proliferation,cloning,migration and invasion of AGS and HGC-27 cells were detected by cell counting,plate cloning assay and Transwell assay,respectively.mRNA and protein expression levels of related genes in gastric cancer cells were detected by qRT-PCR and Western blotting,respectively.Results:Compared with adjacent tissues,the relative expression level of lncRNA DNAH17-AS1 in gastric cancer tissues was significantly increased(P<0.001).The relative expression of lncRNA DNAH17-AS1 in gastric cancer tissues was correlated with TNM stage and lymph node metastasis(P<0.05).The 5-year survival rate of patients with high DNAH17-AS1 expression was significantly lower than that of patients with low DNAH17-AS1 expression(P<0.05).The relative expression level of lncRNA DNAH17-AS1 in plasma of patients with gastric cancer was significantly higher than that of healthy subjects(P<0.01);and the area under the ROC curve of plasma DNAH17-AS1 was 0.721(P<0.01).Compared with the si-NC control group,the proliferation,cloning,migration and invasion of gastric cancer AGS and HGC-27 cells in the siRNA knockout group were significantly decreased(P<0.001);and the relative expression of E-cad mRNA and protein was significantly increased(P<0.01),while the mRNA and protein expression of N-cad,transcription factors Slug,Snail,ZEB1,MMP9,and β-catenin was greatly decreased(P<0.05 or P<0.01).Conclusion:LncRNA DNAH17-AS1 is highly expressed in cancer tissues and plasma of patients with gastric cancer,which is associated with poor prognosis of patients.The decrease of DNAH17-AS1 expression could inhibit the proliferation,clone formation,invasion and migration of gastric cancer cells,which may be related to the regulation of epithelial mesenchymal transformation related molecules expression.