江苏大学学报（医学版）2025，Vol.35Issue(2)：118-124,7.DOI:10.13312/j.issn.1671-7783.y240154
薯蓣皂苷元通过上调胃癌EMT抑制肽表达抑制胃癌细胞的增殖和侵袭
Diosgenin inhibited the proliferation and invasion of gastric cancer cells by up-regulating the expression of inhibitory gastric cancer EMT-related peptide
摘要
Abstract
Objective:To investigate the effects of Diosgenin on the proliferation and invasion of gastric cancer cells and its underlying mechanisms.Methods:Gastric cancer MKN-28 and BGC-823 cells were selected and divided into the following groups:control group(routine culture),solvent group(medium supplemented with ethanol at the same volume as the Diosgenin group),and Diosgenin group(medium supplemented with final concentrations of 0.5,2,and 8 μg/mL Diosgenin).The relative expression of inhibitory gastric cancer EMT-related peptide(IGCE)was detected by quantitative real-time PCR(qRT-PCR).Additionally,gastric cancer cells were divided into control group(routine culture),Lv-control group(medium supplemented with recombinant lentivirus Lv-control),and Lv-IGCE group(medium supplemented with recombinant lentivirus Lv-IGCE).At 72 hours post-viral infection,cell proliferation activity was assessed using CCK-8,and cell invasion was evaluated with Trans-well assay.For another experiment,gastric cancer cells were divided into control group(routine culture)and transfection group(cells transfected with pcDNA-IGCE-aa-GFP).At 48 hours post-transfection,green fluorescent protein(GFP)expression was observed under a microscope.MKN-28 cells transfected with pcDNA-IGCE-aa-GFP were further divided into control group(routine culture),solvent group(medium supplemented with ethanol at the same volume as the Diosgenin group),and Diosgenin group(medium supplemented with final concentrations of 0.5,2,and 8 μg/mL Diosgenin).After 48 hours of co-incubation with Diosgenin,IGCE-aa-GFP fusion protein expression was detected by Western blotting.Finally,gastric cancer cells were divided into control group(routine culture)and Lv-IGCE-smORF group(medium supplemented with lentivirus Lv-IGCE-smORF).At 48 hours post-infection,cells were treated with cycloheximide(CHX),and Krüppel-like factor 4(KLF4)protein expression was detected by Western blotting at 0,0.5,1,2,4,and 8 hours post-treatment.Results:qRT-PCR results showed that,compared with the control group,the relative expression of IGCE in MKN-28 and BGC-823 cells treated with Diosgenin was significantly increased(P<0.05 or P<0.01),in a concentration-dependent manner.Functional assays revealed that,compared with the control group,the proliferation and invasion of MKN-28 and BGC-823 cells in the Lv-IGCE group were markedly inhibited(all P<0.01).The microscopic observation results showed that compared with the control group,there were obvious GFP fluorescence signals in MKN-28 and BGC-823 cells in the transfection group.Western blotting results indicated that IGCE-aa-GFP expression in Diosgenin-treated MKN-28 cells was significantly enhanced(all P<0.01),also in a concentration-dependent manner.After CHX treatment,KLF4 protein expression in Lv-IGCE-smORF group MKN-28 cells was higher than that in the control group at 0.5,1,2,4,and 8 hours(P<0.05 or P<0.01).Conclusion:Diosgenin may inhibit the proliferation and invasion of gastric cancer cells by up-regulating IGCE-aa expression and inhibiting the degradation of KLF4 protein.关键词
胃癌/增殖/侵袭/Krüppel样因子 4/微肽/胃癌EMT抑制肽(IGCE)Key words
gastric cancer/proliferation/invasion/Krüppel-like factor 4(KLF4)/micropeptide/inhibitory gastric cancer EMT-related peptide(IGCE)分类
临床医学引用本文复制引用
孟昊莹,吴冠中,毛竹君..薯蓣皂苷元通过上调胃癌EMT抑制肽表达抑制胃癌细胞的增殖和侵袭[J].江苏大学学报（医学版）,2025,35(2):118-124,7.基金项目
上海市2021年度"科技创新行动计划"医学创新研究专项项目(21MC1930500) （21MC1930500）
