江苏大学学报（医学版）2025，Vol.35Issue(2)：125-132,8.DOI:10.13312/j.issn.1671-7783.y240198
miR-1184对幽门螺杆菌感染胃癌细胞生物学行为的影响
Effect of miR-1184 on biological behavior of gastric cancer cells infected by Helicobacter pylori
摘要
Abstract
Objective:To investigate the expression of microRNA-1184(miR-1184)in MGC-803 cells infected by Helicobacter pylori(Hp),and its effects on the proliferation,metastasis and inflammation of MGC-803 cells infected by Hp.Methods:Fluorescence quantitative PCR was used to detect the expression of miR-1184 in Hp-infected MGC-803 cells.MGC-803 cells were divided into control group(miR-NC group),miR-1184 mimics group(miR-1184 group),Hp infection group(miR-NC+Hp group),miR-1184 mimics combined with Hp infection group(miR-1184+Hp group),inhibitor control group(In-NC group)and miR-1184 inhibitor group(anti-miR-1184 group).The effects of miR-1184 and Hp infection on the proliferation,cell cycle,metastasis and expression levels of related proteins of gastric cancer cells were detected by CCK-8 assay,colony formation assay,cell cycle assay,Transwell assay,wound healing assay and Western blotting assay.Results:Compared to the uninfected group,Hp infected MGC-803 cells produced cytopathic effect,and decreased the expression of miR-1184(P<0.01).After transfection of miR-1184 mimics in MGC-803 cells,miR-1184 expression increased,cell activity decreased,colony formation ability decreased,G1 phase cells increased,S phase and G2 phase decreased,and wound healing ability decreased.In addition,miR-1184 mimics up-regulated the expression level of E-cadherin,decreased the expression level of N-cadherin and Vimentin,inhibited the process of epithelial-mesenchymal transformation(EMT)(P<0.05 or P<0.01).Compared with miR-NC+Hp group,miR-1184+Hp group inhibited MGC-803 cell proliferation,cell cycle,migration ability and EMT effects(P<0.05 or P<0.01).Compared with miR-NC+Hp group,miR-1184+Hp group decreased the mRNA expression of TNF-α,IL-1β,IL-6 and IL-8 in MGC-803 cells,and inhibited the proportion of NF-κB p-p65/NF-κB p65 protein expression(P<0.05).Furthermore,miR-1184 inhibitors promoted the proliferation,cell cycle,cell transfer and EMT process of MGC-803 cells(P<0.05 or P<0.01).Conclusion:The expression of miR-1184 is decreased in Hp-infected gastric cancer cells,and the high expression of miR-1184 reversed the effects of Hp infection on the proliferation,migration and inflammatory response of gastric cancer cells.关键词
miR-1184/幽门螺杆菌/胃癌/细胞周期/迁移/NF-κBKey words
miR-1184/Helicobacter pylori/gastric cancer/cell cycle/migration/NF-κB分类
医药卫生引用本文复制引用
蒋颜材,姚镇东,王秀平,刘云,邵世和,纪筠..miR-1184对幽门螺杆菌感染胃癌细胞生物学行为的影响[J].江苏大学学报（医学版）,2025,35(2):125-132,8.基金项目
国家资助博士后研究人员计划(GZC20230992) （GZC20230992）
江苏省卓越博士后计划(2023ZB180) （2023ZB180）
无锡市卫生健康委员会青年基金资助项目(Q202062) （Q202062）
宜兴市卫生健康委员会重大项目(YXKY202209) （YXKY202209）
江苏大学医教协同重点项目(JDY2023013) （JDY2023013）
