江苏大学学报（医学版）2025，Vol.35Issue(2)：139-144,153,7.DOI:10.13312/j.issn.1671-7783.y240146
肝癌相关成纤维细胞中FXR和TGR5表达与T细胞浸润及预后的相关性分析
Role of FXR and TGR5 in regulating T cell infiltration and prognostic outcomes in liver cancer-associated fibroblasts
摘要
Abstract
Objective:To investigate the regulatory roles of farnesoid X receptor(FXR)and takeda G-protein coupled receptor 5(TGR5)in cancer-associated fibroblasts(CAFs)on T-cell infiltration in hepatocellular carcinoma and their impact on patient prognosis.Methods:Multiplex fluorescence immunohistochemistry(mIHC)was performed on hepatocellular carcinoma tissue microarrays and paired adjacent non-tumor tissues.The HALO digital pathology image analysis system was used to quantitatively assess the expression levels of CD4,CD8,alpha-smooth muscle actin,TGR5,and FXR,obtaining fluorescence intensity values.Bioinformatics and statistical analyses were performed using R software.Results:Analysis using mIHC combined with the HALO digital pathology system revealed no significant correlation between number of CAFs and either T cell infiltration or overall survival in HCC patients(P>0.05).However,low FXR expression in CAFs was associated with significantly reduced T cell infiltration and poorer overall survival compared to high FXR expression(P<0.01).Conversely,high TGR5 expression in CAFs correlated with markedly lower T cell infiltration and worse overall survival than low TGR5 expression(P<0.01).Conclusion:FXR and TGR5 in tumor-associated fibroblasts may play crucial roles in regulating T cell infiltration and influencing the prognosis of hepatocellular carcinoma.关键词
法尼醇X受体(FXR)/takeda G蛋白偶联受体5(TGR5)/癌症相关成纤维细胞/CD4+T细胞/CD8+T细胞Key words
farnesoid X receptor(FXR)/takeda G-protein coupled receptor 5(TGR5)/cancer-associated fibroblasts/CD4+T cell/CD8+T cell分类
基础医学引用本文复制引用
卢美希,姜润秋..肝癌相关成纤维细胞中FXR和TGR5表达与T细胞浸润及预后的相关性分析[J].江苏大学学报（医学版）,2025,35(2):139-144,153,7.基金项目
国家自然科学基金面上项目(82472819 （）
82173100) （）
