江苏大学学报（医学版）2025，Vol.35Issue(2)：154-160,165,8.DOI:10.13312/j.issn.1671-7783.y240135
严氏温阳化瘀利水方改善慢性心衰大鼠心脏功能的作用及可能机制
The mechanism of YAN's Wenyang Huayu Lishui Formula on improving cardiac function in rats with chronic heart failure
摘要
Abstract
Objective:To clarify the effect of YAN's Wenyang Huayu Lishui Formula(YWHLF)on improving cardiac function in rats with chronic heart failure(CHF)and to explore its mechanisms.Methods:The CHF rat model was established through abdominal aortic constriction surgery(AAC)and treated with low(18.2 mg/g)and high(36.3 mg/g)doses of YWHLF for 8 weeks.Hemodynamic indicators were measured using Doppler echocardiography,myocardial histopathology was analyzed with HE staining,myocardial cell apoptosis was detected using TUNEL staining,and plasma levels of B-type natriuretic peptide(BNP),reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD),and cardiac troponin T(cTnT)were measured by ELISA.Additionally,the protein expression of nuclear factor E2-related factor 2(NRF2),heme oxygenase-1(HO-1),and NAD(P)H quinone oxidoreductase 1(NQO1)in myocardial tissue was detected by Western blotting.Pre-treat H9c2 cells with 3%and 8%(v/v)YWHLF and 5 μmol/L NRF2 inhibitor ML385 for 1 h,followed by 24 h of oxygen-glucose deprivation(OGD)treatment.Cell proliferation activity was measured using the CCK-8 assay,and expression of NRF2 protein in nuclear was detected by Western blotting.Additionally,the effect of YWHLF on the binding efficiency of NRF2 protein to antioxidant response elements(ARE)in H9c2 cells was analyzed using the luciferase assay.Results:YWHLF administration increased left ventricular systolic pressure(LVSP)and the maximum rate of left ventricular pressure rise(or fall)(±dp/dtmax)in CHF rats,reduced left ventricular end-diastolic pressure(LVEDP),decreased inflammatory infiltration and cell apoptosis in myocardial tissue,reduced plasma levels of BNP,ROS,MDA,and cTnT,and increased SOD levels.Furthermore,YWHLF upregulated the expression of NRF2,HO-1,and NQO1 proteins in myocardial tissue,with the high-dose group showing significantly better effects than the low-dose group.Cellular experiments indicated that YWHLF pretreatment promoted the proliferation of H9c2 cells under OGD conditions and increased nuclear translocation of NRF2 protein,with the high-dose group showing a significantly stronger effect than the low-dose group.The ML385 reversed the effects of YWHLF.YWHLF significantly enhanced the binding of NRF2 protein to ARE in H9c2 cells.Conclusion:YWHLF can improve cardiac function in CHF rats by activating the NRF2 signaling pathway and inhibiting ischemia-induced myocardial injury.关键词
慢性心力衰竭/心肌细胞损伤/核因子E2 相关因子 2(NRF2)/严氏温阳化瘀利水方/腹主动脉缩窄术Key words
chronic heart failure/myocardial cell injury/nuclear factor E2-related factor 2(NRF2)/YAN's Wenyang Huayu Lishui Formula/abdominal aortic constriction surgery分类
临床医学引用本文复制引用
俞瑞群,叶悦,徐鹏媛,张家美..严氏温阳化瘀利水方改善慢性心衰大鼠心脏功能的作用及可能机制[J].江苏大学学报（医学版）,2025,35(2):154-160,165,8.基金项目
上海市虹口区卫生健康委员会临床重点专科建设(HKLCZD2024B01) （HKLCZD2024B01）
