江苏农业学报2025，Vol.41Issue(2)：242-250,9.DOI:10.3969/j.issn.1000-4440.2025.02.004
萘乙酸诱导玉米瘤黑粉病抗性的生理机制
Physiological mechanism of naphthylacetic acid-induced resistance to com-mon smut in maize
摘要
Abstract
In order to clarify the physiological mechanism of naphthylacetic acid(NAA)-induced resistance to maize smut,in this study,maize inbred lines Ye478 and Qi319 were used as materials to analyze the effects of different concen-trations of NAA on the mycelial growth of Ustilago maydis,the activities of antioxidant enzymes and disease-resistant en-zymes,osmotic adjustment substances and the expression of defense-related genes in maize leaves,and to clarify the mech-anism of NAA-induced plant disease resistance.The results showed that 2.0 mmol/L NAA had a significant inhibitory effect on the pathogen of maize smut,and it had a good induced resistance effect after treating maize plants.Compared with the control groups inoculated with the pathogen alone and sterile water,the antioxidant enzyme activity,disease resistance-related enzyme activity and proline content of maize plants inoculated with 2.0 mmol/L NAA solution+pathogen for 24-48 h showed an overall increasing trend,and the malondialdehyde content was significantly reduced.At the same time,the expression levels of maize-related defense genes SOD,PAL,and LOX were up-regulated.At 48 h after inoculation,the relative expression levels of SOD,PAL and LOX genes in leaves of Ye478 and Qi319 inocu-lated with pathogen+2.0 mmol/L NAA increased by 52.44%and 56.83%,43.57%and 10.69%,47.44%and 81.08%,respectively,compared with those inoculated with pathogen alone.The results of this study provide a basis for the application of exogenous NAA to control maize smut.关键词
玉米/萘乙酸/瘤黑粉病/抗氧化酶/防卫基因Key words
maize/naphthylacetic acid/common smut/antioxidant enzyme/defense gene分类
农业科技引用本文复制引用
高妍婷,赵文清,石菁,王芳,闫新明..萘乙酸诱导玉米瘤黑粉病抗性的生理机制[J].江苏农业学报,2025,41(2):242-250,9.基金项目
国家自然科学基金地区科学基金项目(32060487) （32060487）
甘肃省自然科学基金重点项目(23JRRA1405) （23JRRA1405）
大学生创新创业训练计划(SIETP)项目(202301023) （SIETP）
