江苏农业学报2025，Vol.41Issue(2)：258-267,10.DOI:10.3969/j.issn.1000-4440.2025.02.006
嗜线虫致病杆菌YZ011对鳞翅目昆虫杀虫活性及比较基因组学分析
Insecticidal activity and comparative genomics analysis of Xenorhabdus nematophila YZ011 against lepidopteran insects
摘要
Abstract
Entomopathogenic nematode symbionts are important biological control resources.In order to clarify the in-secticidal toxicity and mechanism of the symbiotic bacteria YZ011 isolated from Steinernema carpocapsae on lepidopteran pests,the whole genome sequencing and comparative genomics analysis of strain YZ011 were further carried out on the basis of measuring the hemocoel injection toxicity of strain YZ011 to Plutella xylostella and Spodoptera frugiperda.The results showed that the mortality rates of Plutella xylostella larvae and Spodoptera frugiperda larvae were 75.0%and 56.7%,respectively,after hemocoel injection of 100 nL 1.0×106 CFU/mL strain YZ011 for 24 h,which were significantly higher than those of phosphate buffered saline(PBS)con-trol.The whole genome sequencing results showed that YZ011 was a nematophagous pathogenic bacterium,and its genome contained 19 secondary metabolite gene clusters.Compared with the most homologous strains YL001 and SⅡ,YZ011 contained 29 specific gene clusters,and 586 virulence factor-related genes were annotated.The results of this study analyzed the molecular characteristics of Xenorhabdus nematophila YZ011 at the genomic level,which was of great significance for the comprehensive utilization of strain YZ011.关键词
嗜线虫致病杆菌/杀虫活性/基因组/次级代谢产物/毒力因子Key words
Xenorhabdus nematophila/insecticidal activity/genome/secondary metabolite/virulence factor分类
农业科技引用本文复制引用
林曼曼,徐健,刘琴,韩光杰,黄立鑫,夏杨,李传明,张楠,陆玉荣,徐彬..嗜线虫致病杆菌YZ011对鳞翅目昆虫杀虫活性及比较基因组学分析[J].江苏农业学报,2025,41(2):258-267,10.基金项目
国家重点研发计划项目(2024YFD1400900) （2024YFD1400900）
江苏省农业科技自主创新基金项目[CX(22)1009] （22）
江苏省国际合作项目(BZ2020039) （BZ2020039）
扬州市科技计划项目(YZ2023244) （YZ2023244）
农业基础性长期性科技工作项目(NAES069AM04) （NAES069AM04）
