江苏农业学报2025，Vol.41Issue(2)：268-275,8.DOI:10.3969/j.issn.1000-4440.2025.02.007
基于RT-RAA和CRISPR/Cas12a的一管式玉米矮花叶病毒快速检测方法
A one-tube rapid detection method for maize dwarf mosaic virus based on RT-RAA and CRISPR/Cas12a
摘要
Abstract
Maize dwarf mosaic virus(MDMV)is an important quarantine virus that seriously affects maize produc-tion.To effectively prevent and control maize dwarf mosaic disease,this study constructed a rapid detection method for MDMV based on reverse transcription-recombinase aided amplification(RT-RAA)technology and the CRISPR/Cas12a system.All reagents were reacted in a single test tube,and the reaction results were detected by lateral flow dipstick(LFD).The optimal reaction conditions screened in this study were as follows:primer addition amount of 2.8 μL,FB re-porter molecule concentration of 100 mol/L,RT-RAA system reaction time of 20 minutes,and CRISPR/Cas12a system re-action time of 20 minutes.The one-tube rapid detection method for maize dwarf mosaic virus based on RT-RAA and CRISPR/Cas12a constructed in this study has strong specificity,high sensitivity,and the experimental materials used are convenient for carrying and transporta-tion,making it suitable for on-site detection.关键词
玉米矮花叶病毒/反转录重组酶介导等温核酸扩增(RT-RAA)技术/CRISPR/Cas12a/侧向流动试纸条Key words
maize dwarf mosaic virus/reverse transcription-recombinase aided amplification(RT-RAA)technology/CRISPR/Cas12a/lateral flow dipstick分类
农业科技引用本文复制引用
侯雨萌,赵振兴,王思元,董铮,胡中泽,周涛,张永江..基于RT-RAA和CRISPR/Cas12a的一管式玉米矮花叶病毒快速检测方法[J].江苏农业学报,2025,41(2):268-275,8.基金项目
国家重点研发计划项目(2021YFD1400100、2021YFD1400103) （2021YFD1400100、2021YFD1400103）
