江苏农业学报2025，Vol.41Issue(2)：286-295,10.DOI:10.3969/j.issn.1000-4440.2025.02.009
高寒黑土区不同轮作模式对马铃薯根际土壤细菌群落结构与功能的影响
Effects of different crop rotation modes on the structure and function of bacterial communities in potato rhizosphere soil in high-altitude black soil areas
摘要
Abstract
To clarify the effects of different rotation patterns on bacterial communities in potato rhizosphere soil,six planting patterns of potato-wheat-sugar beet-potato(A treatment),potato-milk thistle-wheat-potato(B treat-ment),wheat-sugar beet-wheat-potato(C treatment),oil-seed rape-wheat-sugar beet-potato(D treatment),wheat-oilseed rape-barley-potato(E treatment)and oilseed rape-wheat-potato-potato(CK,control)were studied.The high-throughput sequencing technology of bacterial 16S region was used to compare and analyze the effects of six local planting patterns on the structure and function of bacterial com-munities in potato rhizosphere soil.The results showed that a total of 36 420 amplicon sequence variations(ASVs)were obtained from the soil samples under the six rotation patterns,which were divided into 45 phyla,119 classes,298 orders,451 families,790 genera and 357 species.The dominant bacteria in potato rhizosphere soil under differ-ent rotation modes were Proteobacteria,Acidobacteria,Bacteroidetes,Gemmatimonadota,Actinobacteria,Plancto-mycetota,Verrucomicrobiota,Chloroflexi,Myxococcota and Patescibacteria.However,the relative abundance of the dominant bacteria was different under different rotation patterns.Compared with CK,rotation significantly increased the relative abundance of Acidobacteria and Gemmatimonadota,while the relative abundance of Actinobacteria de-creased significantly.In addition,the relative abundance of Lysobacter and functional bacteria related to carbon and ni-trogen increased.The results of inter-group community difference analysis(LEfSe)showed that 38 differential species in the bacterial community were identified.Through gene function prediction,it was found that the relative abundance of metabolism in primary function was the highest(62.04%-62.46%).There were 19 categories of secondary func-tional metabolic pathways with relative abundance greater than 1.0%,among which carbohydrate metabolism had the highest relative abundance(12.93%-13.29%).In summary,rotation changed the soil bacterial community structure and increased the dominant functional bacteria in soil bacteria.In this study,the rotation mode of wheat-sugar beet-wheat-potato had the best effect,which was more suitable for the sustainable development of potato industry in high-altitude black soil areas.关键词
高寒黑土区/轮作模式/马铃薯/根际土壤/细菌群落结构/细菌群落功能Key words
high-altitude black soil areas/rotation pattern/potato/rhizosphere soil/bacterial community struc-ture/bacterial community function分类
农业科技引用本文复制引用
王籽懿,韩伟,金鸿飚,黄修梅,李明..高寒黑土区不同轮作模式对马铃薯根际土壤细菌群落结构与功能的影响[J].江苏农业学报,2025,41(2):286-295,10.基金项目
内蒙古自治区重点研发和成果转化计划项目(2022YFDZ-0010) （2022YFDZ-0010）
河北省科技计划结转项目(21326320D) （21326320D）
内蒙古自治区农牧厅基层农业推广体系改革与建设补充及重大农业技术协同推广资金项目 （）
