菌物学报Issue(3)：37-49,13.DOI:10.13346/j.mycosystema.240141
灵芝优良品种全基因组分子标记的建立
Establishment of molecular markers in the whole genome of Ganoderma lingzhi elite varieties
摘要
Abstract
Ganoderma lingzhi 13 is an excellent cultivated variety with superior agronomic character.The mononucleate strain,G.lingzhi 13-5,as assembled into a complete genome by three-generation HiFi sequencing and Hi-C sequencing,and five replicated bi-nucleate strains were subjected to high throughput sequencing by Illumina,and then the genome of G.lingzhi 13-5 was used as a reference for SNP calling to establish the molecular markers based on the whole genome of G.lingzhi 13.The genome contains 13 chromosomes with a total of 45.73 Mb.After the SNP calling of five replicate strains were de-emphasized and merged,319 074 shared heterokaryotic allelic difference sites were obtained as a molecular marker library for the identification of G.lingzhi 13 strains.Seven post-fruiting strains were randomly selected from the strain library.It was found that the percentage of allelic difference sites of the post-fruiting strains shared with G.lingzhi 13 ranged from 22.20％to 45.18％,being far inferior to threshold value,indicating that these strains were different from G.lingzhi 13 and they were not the same strains.In this study,molecular markers based on the whole genome of G.lingzhi 13 and its heterokaryotic allelic differentiation sites were established,which can accurately differentiate G.lingzhi 13,a superior cultivated variety.关键词
三代HiFi测序/SNP calling/品种鉴别/品种保护Key words
three generations of HiFi sequencing/SNP calling/variety identification/variety protection引用本文复制引用
刘逸婷,蒋晓涵,杨春艳,陈健辉,王车昭,吕晓萌,杨治康,邓优锦,吴小平..灵芝优良品种全基因组分子标记的建立[J].菌物学报,2025,(3):37-49,13.基金项目
福建省科技重大专项(2022NZ029015)This work was supported by the Fujian Provincial Major National Research and Development Project(2022NZ029015). （2022NZ029015）
