菌物学报Issue(3)：50-61,12.DOI:10.13346/j.mycosystema.240252
糙皮侧耳AQPs基因家族鉴定与表达分析
Identification and expression analyses of the aquaporin gene family in Pleurotus ostreatus
摘要
Abstract
The aquaporin(AQP)gene family plays a crucial role in water transport and various physiological processes in fungi.In this study,the members of the AQP family in Pleurotus ostreatus(PoAQPs)were systematically identified using bioinformatics approaches,and their physicochemical properties,subcellular localization,chromosomal distribution,phylogenetic relationships,conserved motifs,and cis-regulatory elements of the promoters were analysed.The relative expression levels of PoAQPs were evaluated in various growth stages and under waterlogging stress conditions using RT-qPCR.As a result,five members of the aquaporin(PoAQPs)gene family in the genome of Pleurotus ostreatus were identified,which were categorized into two subfamilies,classical aquaporins(AQPs)and aquaglyceroporins(AQGPs).All members contained the MIP superfamily domain and exhibited the characteristic tertiary structure of classical water channel proteins.The five PoAQPs genes were distributed among four chromosomes,and subcellular localization predictions indicated that these genes were located at the cell membrane.Members within the same subfamily shared similar conserved motifs.The promoter regions were enriched with numerous regulatory elements related to hormonal responses and abiotic stress.RT-qPCR results revealed significant differences in the expression levels of PoAQPs genes in various growth stages and in response to waterlogging stress,with particularly high expression during the fruiting body stage and after 8 hours of waterlogging stress.It is suggested that PoAQPs are involved in the regulation of the growth,the development of Pleurotus ostreatus as well as the responses to abiotic stress.These findings offer valuable theoretical insights into the mechanisms of water regulation in Pleurotus ostreatus and give support to the breeding of stress-resistant varieties.关键词
糙皮侧耳/水通道蛋白/基因家族/生物信息学/表达分析Key words
Pleurotus ostreatus/aquaporins/gene family/bioinformatics/expression analysis引用本文复制引用
胡继鹏,鲍大鹏,邹根,高贺宇,尚俊军,唐利华..糙皮侧耳AQPs基因家族鉴定与表达分析[J].菌物学报,2025,(3):50-61,12.基金项目
国家自然科学基金(32172649) （32172649）
山东重点研发计划(2022CXGC010610)This work was supported by the National Natural Science Foundation of China(32172649)and the Major Innovation Project of Shandong Province(2022CXGC010610). （2022CXGC010610）
