菌物学报Issue(3):62-75,14.DOI:10.13346/j.mycosystema.240255
野生巨大侧耳生物学特性及驯化栽培
Biological characteristics and domestication of wild Pleurotus giganteus
摘要
Abstract
A wild strain AH 150 of Pleurotus collected from Qiyun Mountain of Anhui Province was isolated and identified as Pleurotus giganteus based on morphological observation and ITS sequence analyses.The suitable carbon source,nitrogen source,temperature and pH for the mycelial growth were screened by single factor and orthogonal test.The results showed that the optimum carbon source and nitrogen source were fructose and peptone respectively.Under the optimum temperature of 30 ℃ and optimum pH of 8,the relative growth rate was the fastest and the mycelium was most robust and dense.The substrate for domesticated cultivation was sawdust 52%,corn cob 25%,wheat bran 20%,lime 2%and gypsum 1%.After spawning and covering soil,the air humidity was kept at more than 80%.The primordium appeared on the 11th day.Under humidification of 85%-90%,the fruiting body appeared in 4-5 d.This research provides a reference for extended development and utilization of P.giganteus AH150.关键词
巨大侧耳/食药用真菌资源/生物学特性/驯化栽培Key words
Pleurotus giganteus/edible and medicinal fungal resources/biological characteristics/domestication and cultivation引用本文复制引用
李曼玲,朱安红,马国营,屈直,卢雪花,马海霞,刘振东..野生巨大侧耳生物学特性及驯化栽培[J].菌物学报,2025,(3):62-75,14.基金项目
海南省重点研发计划-海南国家公园研究院资助项目(ZDYF2023RDYL01,KY-24ZK02) (ZDYF2023RDYL01,KY-24ZK02)
五指山市生态科技特派员项目(WZSKTPXM202211) (WZSKTPXM202211)
中央级公益性科研院所基本科研业务费专项(1630052022003)This work was supported by the Hainan Institute of National Park and Hainan Province Science and Technology Special Fund(ZDYF2023RDYL01,KY-24ZK02),the Ecological Science and Technology Project of Wuzhishan City(WZSKTPXM202211),and the Central Public-Interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630052022003). (1630052022003)