菌物学报Issue(3)：76-100,25.DOI:10.13346/j.mycosystema.240256
皱皮粗柄白蚁伞子实体和蚁巢的关联物质分析及氨基酸糖类促生作用初探
Analyses of substances associating Termitomyces robustus fruiting bodies with termite nests and preliminary studies on growth-promoting effect of amino acids and sugars
摘要
Abstract
Termitomyces robustus is a rare wild fungus in Yunnan,eciprocally symbiotic with termites in termite nests,and the development of its fruiting bodies remains mysterious.In this study liquid chromatography-mass spectrometry(LC-MS)was used to analyze the metabolite profiles of T.robustus fruiting bodies and their growth substrate termite nests.In total,2 023 metabolites were detected in the fruiting bodies(JZ)and the termite nests(YC),including 284 JZ-specific substances(JZS),317 YC-specific substances(YCS),and 322 common significantly differential substances(GDS).Citing the compounds with relative content≥0.5 as instance,Cytoscape(v3.10.0)software was used to show the significant correlation among 39 substances in 43 JZS and YCS and 19 substances in 29 GDS.A total of 40 JZS,YCS and GDS substances in 18 common pathways was enriched by KEGG,which were associated through three major categories of pathways,ABM(amino acid biosynthesis and metabolism),BSM(biosynthesis of secondary metabolites)and ABC(ATP-binding cassette)transporters.Among the 18 pathways,12 were related to ABM and 2 were related to glucose metabolism in ABC transporters.Nine amino acids(phenylalanine,isoleucine,tyrosine,proline,threonine,valine,tryptophan,leucine,and glutamic acid)involved in the ABM pathway,three sugars(sucrose,sorbitol,and trehalose)involved in ABC transporters,and termite water extract were selected to study the growth-promoting effect of purely cultured T.robustus mycelia,and the results showed that the addition of 0.2-0.6 g 9 complex amino acids,20 g glucose+5 g sucrose or sorbitol or trehalose,and 50-150 mL termite nest water extracts in per liter of basal media significantly promoted the growth of mycelia.However,the promoting effect of adding 150 mL termite nest water extracts on mycelial growth was significantly better than that of complex amino acid and sugar treatment(0.6 g complex amino acid+20 g glucose+5 g trehalose)and compound amino acid treatment(0.6 g complex amino acid).284 JZS,317 YCS and 322 GDS,especially the above 98 substances were significantly correlated to the fruiting bodies and termite nests,and it was speculated that they played an important role in regulating the development of the fruiting bodies of Termitomyces.The complex amino acid,sorbitol or trehalose had a significant effect on promoting the growth of purely cultured mycelia,but they were inferior to the water extract of termite nest.Further analysis of signal molecules related to the growth and development of Termitomyces fruiting bodies is needful,for the purpose of exploring the symbiotic mechanism of Termitomyces and termites,and large-scale artificial cultivation of Termitomyces.关键词
皱皮粗柄白蚁伞/蚁巢/关联物质/关联通路/复合氨基酸/糖类/促生作用Key words
Termitomyces robustus/termite nest/associated substance/associated pathways/complex amino acids/sugars/growth promoting effect引用本文复制引用
王成凤,刘夏,张曦予,董杰,张实润,杨晓敏,吕为,刘佳艳,李宗菊..皱皮粗柄白蚁伞子实体和蚁巢的关联物质分析及氨基酸糖类促生作用初探[J].菌物学报,2025,(3):76-100,25.基金项目
国家自然科学基金(31560575) （31560575）
云南省自然科学基金(2014FA020)This work was supported by the National Natural Science Foundation of China(31560575)and the Natural Science Foundation of Yunnan Province(2014FA020). （2014FA020）
