菌物学报Issue(3)：101-110,10.DOI:10.13346/j.mycosystema.240251
银耳多糖对肠道屏障的影响及作用机制
Effects of Tremella fuciformis polysaccharides on intestinal barrier and its functional mechanism
摘要
Abstract
Palmitic acid was used to stimulate human colon cancer epithelial cells Caco-2 to construct a tight-junction protein injury model under high fat diet for studying the effect of Tremella fuciformis polysaccharides on intestinal barrier and its functional mechanism.By measuring cell viability,cell integrity,redox level,inflammatory factor content,tight junction gene,protein expression level,and inflammatory signaling pathway expression level,it was found that 400 μg/mL T.fuciformis polysaccharides was non-toxic to Caco-2 cells,and could increase SOD and CAT content and decrease MDA content,inhibit the release of pro-inflammatory factors IL-1β,TNF-α and IL-6,enhance the mRNA and protein expression levels of tight junction proteins Claudin-1,Occludin and ZO-1,and down-regulate the secretion of pro-inflammatory factors by inhibiting the inflammatory signaling pathway of TRL4/MyD88/NF-κB.T.fuciformis polysaccharides maintained the intestinal barrier by means of anti-oxidation,inhibiting the secretion of inflammatory factors and elevating the expression of tight junction protein.关键词
银耳多糖/棕榈酸/肠道炎症/肠道屏障Key words
Tremella fuciformis polysaccharides/palmitic acid/inflammatory bowel disease/gut barrier引用本文复制引用
董茜,张仕林,谢丽源,张谦,舒雪琴,何晓兰,彭卫红,许瀛引..银耳多糖对肠道屏障的影响及作用机制[J].菌物学报,2025,(3):101-110,10.基金项目
国家自然科学基金(32402653) （32402653）
四川省自然科学基金(2023NSFSC1249) （2023NSFSC1249）
四川省财政自主创新专项(2022ZZCX096)This work was supported by the National Natural Science Foundation of China(32402653),the Sichuan Natural Science Foundation(2023NSFSC1249),and Sichuan Province Financial Independent Innovation Project(2022ZZCX096). （2022ZZCX096）
