菌物学报Issue(3)：124-136,13.DOI:10.13346/j.mycosystema.240261
富硒刺芹侧耳硒肽的提取工艺及其抗氧化活性
Extraction process and antioxidant activities of selenopeptides from selenium-enriched Pleurotus eryngii
摘要
Abstract
Selenium is a crucial trace element in human physiology,playing an essential role in life-sustaining processes through selenoproteins and selenopeptides.In this study,by combining alkali extraction with acid precipitation,selenoproteins were successfully isolated from fruiting bodies of Pleurotus eryngii.Single-factor test was conducted to determine the optimal parameters for enzymatic hydrolysis.Box-Behnken design was applied to further refine the selenopeptide extraction process.Furthermore,four indexes of DPPH,ABTS,hydroxyl radical scavenging,and FRAP were compared and analyzed with ordinary peptides.The results showed that under optimized conditions,the selenium content of the extracted selenopeptides reached 226.33 mg/mL,at enzyme concentration of 8 000 U/g,hydrolysis temperature of 60 ℃,and hydrolysis time of 73 minutes.The selenopeptide of selenium-enriched P.eryngii fruiting bodies exhibited significantly greater antioxidant activity than ordinary peptides in DPPH radical scavenging rate,ABTS radical scavenging rate,hydroxyl radical scavenging rate,and FRAP total antioxidant capacity,likely due to the synergistic effect between selenium content and polypeptides,enhancing the antioxidant activity of selenopeptides.Preparation and application of selenopeptides from selenium-enriched P.eryngii fruiting bodies are in need of further study.关键词
刺芹侧耳/硒肽/硒含量/抗氧化活性Key words
Pleurotus eryngii/selenopeptide/selenium content/antioxidant activity引用本文复制引用
陈莉莉,吴惠华,徐舒敏,朱志远,谢宝贵,陈炳智..富硒刺芹侧耳硒肽的提取工艺及其抗氧化活性[J].菌物学报,2025,(3):124-136,13.基金项目
国家重点研发计划(2022YFD1200604) （2022YFD1200604）
福建农林大学科技创新专项基金(CXZX2020116A,KFB23134A)This work was supported by the National Key Research and Development Program(2022YFD1200604)and the Special Fund for Scientific and Technological Innovation of Fujian Agriculture and Forestry University(CXZX2020116A,KFB23134A). （CXZX2020116A,KFB23134A）
