棉花学报2025，Vol.37Issue(1)：13-24,12.DOI:10.11963/cs20240062
新型氮肥增效剂N-life Ⅱ对棉田土壤氮素供给及相关酶活性的影响
Effects of a new nitrogen fertilizer synergist N-life Ⅱ application on soil nitrogen supply and related enzyme activities in cotton fields
摘要
Abstract
[Objective]This study aimed to clarify the effect of N-life Ⅱ(the main active ingredient is nitrapyrin)on soil nutrient contents and soil nitrogen(N)cycle-related enzyme activities under different application levels of N,so as to provide a basis for the application of N-life Ⅱ in cotton production.[Methods]Field experiments were carried out at Sanya,Hainan province in 2021 and 2022 with Zheda 12 as the experimental material.The field experiment was designed with two factors split-pot.The main plot was N-life Ⅱ application level:22.5 kg and 0 kg(control)per hectare,respectively;the secondary plot was pure N application level:285.0 kg(conventional level),256.5 kg(10％N reduction),228.0 kg(20％N reduction),and 199.5 kg(30％N reduction,2022 only)per hectare,respectively.N,phosphorus and potassium contents as well as urease,ammonia monooxygenase(AMO),hydroxylamine oxidoreductase(HAO),nitrite oxidoreductase(NXR),nitrate reductase(NR),and nitrite reductase(NiR)activities in soil were analyzed at the seedling stage,flowering and boll setting stage,and boll opening stage of cotton under different treatments.[Results]Compared with the respective control treatments at the same N application level,soil ammoniacal N content increased at the flowering and boll setting stage and boll opening stage under the N-life Ⅱ treatment;soil nitrate N content decreased at the seedling stage and flowering and boll setting stage,and increased at the boll opening stage;and there were no significant differences in the contents of soil total N,P2O5 and K2O at the seedling stage,flowering and boll setting stage,and boll opening stage.Compared with the mean values of all control treatments,the average soil total N content under N-life Ⅱ treatments was significantly increased by 6.10％to 6.63％at the boll opening stage;the average soil P2O5 and K2O contents under N-life Ⅱ treatments were significantly reduced during the flowering and boll setting stage and boll opening stage.Application of N-life Ⅱ reduced the activities of soil urease,AMO,NR,and NiR at the seedling stage and flowering and boll setting stage;reduced soil NXR activity at the seedling stage;enhanced soil urease activity at the boll opening stage;and enhanced soil NiR activity at the boll opening stage under the normal N application level;while had no significant effect on soil HAO activity at different growth stages.[Conclusion]Under different application levels of N,N-life Ⅱ reduced soil nitrate N content at the seedling stage and flowering and boll setting stage,and increased soil ammoniacal N content at the flowering and boll setting stage and boll opening stage by inhibiting the activities of urease,AMO,NXR,NR,and NiR in soil.关键词
棉花/N-life Ⅱ/硝化抑制剂/土壤养分含量/土壤酶活性Key words
cotton/N-life Ⅱ/nitrification inhibitor/soil nutrient content/soil enzyme activity引用本文复制引用
石钇琦,祝越熠,马心雨,祝水金,赵天伦..新型氮肥增效剂N-life Ⅱ对棉田土壤氮素供给及相关酶活性的影响[J].棉花学报,2025,37(1):13-24,12.基金项目
海南省重点研发计划(SQ2021XDNY0696) （SQ2021XDNY0696）
江苏省现代作物生产协同创新中心 （）
