棉花学报2025，Vol.37Issue(1)：59-70,12.DOI:10.11963/cs20240066
基于线粒体CO Ⅰ基因的湖南省棉叶蝉种群遗传多样性研究
Study on the genetic diversity of Amrasca biguttula population in Hunan Province based on mtDNA CO Ⅰ gene
摘要
Abstract
[Objective]The occurrence of Amrasca biguttula in the cotton field of Hunan Province is increasingly growing.The purpose of this study was to analyze the genetic diversity and genetic differentiation of A.biguttula in Hunan Province.[Methods]In 2023,14 geographical population samples of A.biguttula were collected in Hunan Province.Based on the mitochondrial DNA(mtDNA)cytochrome c oxidase subunit Ⅰ(CO Ⅰ)gene sequences obtained by polymerase chain reaction(PCR),the genetic diversity,genetic differentiation,population dynamics,and systematic evolution of the 14 geographical populations of A.biguttula in Hunan Province were analyzed using MEGA 7.0,DnaSP 6.1,Arlequin 3.5,Network 10.2,and other softwares.[Results]The mtDNA CO Ⅰ sequences of 568 bp were obtained from 140 individuals by PCR.A total of 14 haplotypes(Hap1-Hap14)were detected,among which Hap1 was shared by all populations.Hapl had a frequency of 87.86％,and was the original haplotype.The haplotype diversity index of the whole A.biguttula community was 0.226 95,the nucleotide diversity was 0.000 52,and the average number of nucleotide difference was 0.296 25.The genetic distance between haplotypes ranged 0.001 76 to 0.007 09.The whole A.biguttula community had a low degree of genetic differentiation(the genetic differentiation coefficient is 0.016 84),and active gene exchange(the gene flow is 29.19).Molecular variance analysis(AMO VA)results indicated that genetic variation mainly originated within the population.The Tajima's D,Fu and Li's D,and Fu and Li's F neutral tests for the whole A.biguttula community were significantly negative,suggesting that the A.biguttula population in Hunan Province is undergoing expansion.[Conclusion]The geographical population of A.biguttula in Hunan Province showed low genetic diversity,active gene exchange,and low genetic differentiation;and the total population is experiencing obvious expansion.Active monitoring and control measures should be taken to ensure the healthy development of cotton industry in Hunan Province.关键词
棉叶蝉/线粒体CO Ⅰ基因/地理种群/遗传多样性/湖南省Key words
Amrasca biguttula/mtDNA CO Ⅰ gene/geographical population/genetic diversity/Hunan Province引用本文复制引用
刘冰蕾,王永波,张正云,杨彬,李彩红..基于线粒体CO Ⅰ基因的湖南省棉叶蝉种群遗传多样性研究[J].棉花学报,2025,37(1):59-70,12.基金项目
湖南省科技创新计划(2023NK2015) （2023NK2015）
国家现代农业产业技术体系(CARS-15-38) （CARS-15-38）
