生态与农村环境学报2025，Vol.41Issue(3)：378-388,11.DOI:10.19741/j.issn.1673-4831.2023.1078
3株解磷菌的解磷机理及其对杉木幼苗的促生效应
The Phosphate-solubilizing Mechanism of Three Phosphate-solubilizing Bacteria and Their Promoting Effect on Cunninghamia lanceolata
摘要
Abstract
To elucidate the phosphate-solubilizing mechanisms of phosphate-solubilizing bacteria(PSB)in the rhizosphere of Cunninghamia lanceolata in the red soil region of southern China and validate their growth potential,this study aims to provide data support for the development and utilization of these strains.Three PSB strains,namely W1(Acinetobacter sp.,A),W10(Acinetobacter sp.,B),and Y9(Klebsiella sp.,C),isolated from the rhizosphere of Cun-ninghamia lanceolata,were employed as test strains.The soluble phosphorus content was determined for inorganic PSB strains under various insoluble inorganic phosphorus sources[Ca3(PO4)2,FePO4,AlPO4]and for organic PSB strains under different insoluble organic phosphorus sources(calcium phytate,lecithin).A preliminary analysis of the PSB strains'mechanisms was conducted by measuring D600,soluble phosphorus content,pH,types and contents of organic acids,and enzymatic activity during the phosphate-solubilization process.Additionally,a pot experiment with Cunning-hamia lanceolata was performed to elucidate the effects of different strain combinations(A,B,C,AB,AC,BC,ABC)on the growth index,nutritional index,and soil nutrient content of Cunninghamia lanceolata seedlings,thereby verifying their growth-promoting abilities.The key findings are as follows:(1)The inorganic PSB strains W1 and W10 exhibited the optimal dissolution effect on Ca3(PO4)2,primarily secreting organic acids to solubilize unavailable phosphorus.The pH of the bacterial solution was significantly negatively correlated with available phosphorus content(P<0.01).In contrast,the organic PSB strain Y9 primarily relied on acid phosphatase and alkaline phosphatase for hydrolysis.(2)The seedling height and ground diameter growth in the late stages of the ABC treatment with compound bacterial fertilizer were signifi-cantly higher than those of other treatments(P<0.05).Compared to the control(CK),the growth increasing rates were 20.5%(seedling height)and 33.2%(ground diameter),respectively.(3)The aboveground biomass of the ABC treat-ment was 49.4%higher than that of CK(P<0.05),whereas the underground part growth was less than that of the BC treatment.(4)Under the mixed bacteria ABC treatment,plant nutrient content(total nitrogen,total phosphorus,total po-tassium,total organic carbon)and soil nutrient content(soil available phosphorus,available potassium,hydrolyzed nitro-gen)significantly increased compared to CK.Each strain demonstrated varying abilities to solubilize insoluble phosphorus in different forms.During the hydrolysis of inorganic phosphorus,W1 and W10 primarily secreted organic acids,whereas Y9 primarily relied on enzymatic secretion for organic phosphorus solubilization.Regarding growth promotion,the three strains all positively influenced plant growth and soil nutrient content to varying degrees,with the combined benefit of strains being significantly superior to that of a single strain.The results of this study provide data support and a research basis for improving the available phosphorus content of Cunninghamia lanceolata soil and plant growth index through the application of microbial fertilizer.Furthermore,the results of this study preliminarily validate the feasibility of enhancing the nutritional environment of forest trees by using functional bacterial fertilizer.关键词
解磷菌/解磷机理/促生作用/杉木Key words
phosphate-solubilizing bacteria/phosphate solubilization mechanism/promoting effect/Cunninghamia lan-ceolata分类
农业科学引用本文复制引用
刘浩蓝,丁珊珊,包小春,徐乾乐,赵君,朱清波,林晗,李键..3株解磷菌的解磷机理及其对杉木幼苗的促生效应[J].生态与农村环境学报,2025,41(3):378-388,11.基金项目
国家自然科学基金(32071753) （32071753）
福建省林业局种业创新与产业化工程(2021-2025年)林业项目(KLb21009B) （2021-2025年）
