南京中医药大学学报2025，Vol.41Issue(3)：306-312,7.DOI:10.14148/j.issn.1672-0482.2025.0306
涤痰汤靶向PDE4B减轻卒中后中性粒细胞浸润及神经血管单元损伤
Targeting PDE4B with Ditan Decoction Inhibits Neutrophil Infiltration to Mitigate Neurovascular Unit Injury
摘要
Abstract
OBJECTIVE To investigate the neuroprotective effects of Ditan Decoction(DTD)on ischemic stroke.METHODS A mouse middle cerebral artery occlusion(MCAO)model was used to induce cerebral ischemia and assess the role of DTD in post-stroke NVU injury.DTD was gavaged once a day for 3 days after MCAO.Transwell neutrophil chemotaxis assay was used to explore the role of DTD in the neutrophil chemotaxis.RESULTS In the MCAO model,DTD treatment significantly reduced infarct volume(P<0.01)and attenuated blood-brain barrier disruption,as evidenced by decreased IgG leakage and preserved laminin expression(P<0.05).Furthermore,DTD suppressed neutrophil infiltration into ischemic brain tissue,as demonstrated by reduced neutrophil elastase(P<0.01)and myeloperoxidase(P<0.05)levels.Mechanistically,DTD inhibited neutrophil chemotaxis in a dose-dependent manner and downregulated phosphodiesterase 4B(PDE4B),a key regulator of neutrophil migration(P<0.05).Molecular docking analysis i-dentified four active DTD components-apigenin,vitexin,chlorogenic acid,and orientin-with strong binding affinities to PDE4B(bind-ing energies<-5 kcal·mol-1),suggesting their potential role in mediating DTD's therapeutic effects.CONCLUSION These find-ings highlight DTD as a promising intervention for ischemic stroke,targeting NVU preservation and PDE4B-dependent neutrophil mod-ulation.关键词
涤痰汤/中性粒细胞浸润/缺血性卒中/神经血管单元/PDE4B/髓过氧化物酶/分子对接Key words
Ditan Decoction/neutrophil infiltration/ischemic stroke/neurovascular unit/PDE4B/myeloperoxidase/molecular docking分类
医药卫生引用本文复制引用
俞书红,刘思洁,朱佳怡,范玲,顾佳美,黄昊,罗毅..涤痰汤靶向PDE4B减轻卒中后中性粒细胞浸润及神经血管单元损伤[J].南京中医药大学学报,2025,41(3):306-312,7.基金项目
吴中区科技计划项目(WZYW2023003) （WZYW2023003）
苏州市中西医结合医院科技计划项目(YJ2021002) （YJ2021002）
