农业工程学报2025，Vol.41Issue(3)：126-135,10.DOI:10.11975/j.issn.1002-6819.202409025
氮肥联合EDDS对三叶鬼针草修复镉污染土壤的影响
Effects of the nitrogen fertilizer combined with EDDS on the remediation of cadmium contaminated soil by Bidens pilosa L.
摘要
Abstract
In order to improve the remediation efficiency of Bidens pilosa L.on farmland heavy metal cadmium(Cd)-contaminated soil,based on pot experiments,in 15 mg/kg Cd-contaminated soil.The effects of three concentrations(25,50 and 75 mg/kg,based on nitrogen)of nitrate nitrogen(NO3--N),ammonium nitrogen(NH4+-N)and amide nitrogen(amide-N)combined with 1 mmol/L[S,S]-ethylenediamine disuccinate acid(EDDS)on the growth,Cd accumulation and active oxygen metabolism of Bidens pilosa L.were studied,and the principal component analysis and correlation analysis between different indexes were carried out in order to provide method reference and theoretical basis for the remediation and treatment of typical high concentration Cd contaminated farmland soil.The results showed that the combined treatment of NO3--N and EDDS significantly improved the growth and Cd accumulation of Bidens pilosa L.in Cd-contaminated soil,compared to NH4+-N or amide-N.Among all treatments,the combined treatment of 75 mg/kg NO3--N and EDDS significantly promoted the growth of Bidens pilosa L.in Cd-contaminated soil,with increases of 27.0%,45.6%,25.5%,and 38.1%in fresh mass of shoot,dry mass of shoot,fresh mass of root,and dry mass of root compared to the Cd+EDDS treatment,and increases of 41.7%,24.0%,20.5%,and 19.5%,respectively,compared to the Cd treatment.Under different treatments,the Cd transport capacity of Bidens pilosa L.was weak,and the roots of the plants accumulated more Cd than the shoots.The combined treatment of 50 mg/kg NO3--N and EDDS significantly increased the enrichment of Cd by Bidens pilosa L.in Cd-contaminated soil,with increases of 45.8%,59.5%,46.1%,and 46.7%in Cd accumulation of shoot and root,bioconcentration factor(BCF),and phytoextraction efficiency(PEE)compared to the Cd+EDDS treatment,and increases of 125.7%,181.1%,125.5%,and 97.4%,respectively,compared to the Cd treatment.In addition,the effects of the three forms of nitrogen fertilizers combined with EDDS on the reactive oxygen metabolism of Bidens pilosa L.in Cd-contaminated soil varied.Among them,under the treatment of 50 mg/kg NO3--N combined with EDDS,peroxidase(POD)and superoxide dismutase(SOD)played a leading role in the antioxidant enzyme system.POD and SOD were increased by 8.8%and 36.9%,respectively,compared to the Cd+EDDS treatment,and 20.7%and 46.4%,respectively,compared to the Cd treatment;the contents of reduced glutathione(GSH)and proline(Pro)in plants increased by 12.7%and 86.3%,respectively,compared to the Cd+EDDS treatment,and increased by 7.3%and 70.7%,respectively,compared to the Cd treatment.Under this treatment,the plants alleviated the membrane lipid peroxidation damage caused by Cd stress by enhancing the activity of antioxidant enzymes and increasing the content of antioxidants,as a result,the level of hydrogen peroxide(H2O2)was significantly lower than that of Cd treatment and the tolerance of plants to Cd was improved.Considering all factors,the combination treatment of 50 mg/kg NO3--N and 1 mmol/L EDDS can improve the remediation efficiency of Bidens pilosa L.in Cd-contaminated soil.It is suggested that the combined remediation technology should be used to regularly plant and harvest Bidens pilosa L.with 67 days as a cycle in the growth period of this experiment,and the soil Cd content should be reduced by continuous cropping.关键词
土壤/植物修复/三叶鬼针草/EDDS/氮肥/Cd胁迫Key words
soils/phytoremediation/Bidens pilosa L./EDDS/nitrogen fertilizer/Cd stress分类
环境科学引用本文复制引用
曹渤,陈银萍,郑彦,卢誉之,李倩,孙勇,袁巧玲..氮肥联合EDDS对三叶鬼针草修复镉污染土壤的影响[J].农业工程学报,2025,41(3):126-135,10.基金项目
国家自然科学基金项目(32260316) （32260316）
自然资源部黄河上游战略性矿产资源重点实验室2023年度开放基金项目(YSMRKF202306) （YSMRKF202306）
