热带作物学报2025，Vol.46Issue(3)：524-533,10.DOI:10.3969/j.issn.1000-2561.2025.03.002
澳洲坚果锌指蛋白基因MiZFP11响应干旱和高温胁迫的功能分析
Functional Analysis of Zinc Finger Protein Gene MiZFP11 from Macadamia in Response to Drought and High Temperature Stresses
摘要
Abstract
CCCH-type zinc finger protein plays an important regulatory role in plant growth,development,and response to stress.In this study,a zinc finger protein gene MiZFP11 was isolated from macadamia(Macadamia integrifolia)va-riety JW and Guire No.1 by RT-PCR,and the structural characteristics and subcellular localization of its encoded protein was analyzed.Expression levels of MiZFP11 in drought and high temperature treatments and the tissues of macadamia were investigated by quantitative real-time PCR.RT-PCR cloning showed that a new gene MiZFP11 from macadamia was highly homologous to CCCH zinc finger protein genes,with GenBank registration number MT332641.Bioinfor-matics analysis indicated that MiZFP11 belonged to C3H13 subclass zinc finger protein with an LCCL domain at rela-tively conservative C-terminus.Analysis of protein basic properties indicated that MiZFP11 was an unstable hydrophilic protein without signal peptide and transmembrane segment,which was mainly phosphorylated with serine.Protein structure analysis showed that the secondary and tertiary structures of MiZFP11 were mainly composed of random coil,α-helix and extended strand.Transient expression of tobacco leaves and the confocal microscopy detection indicated that MiZFP11 was localized in the nucleus,which was consistent with the subcellular localization predicted results.qRT-PCR analysis showed that expression of MiZFP11 gene was highest in leaves of macadamia variety JW and Guire No.1,next was in roots,small flowers and small fruits,with the lowest in stems.Under drought stress for 1-36 hours,expression level of MiZFP11 gene in leaves increased with an"up-down-up-down"pattern in JW and an"up-down"pattern in Guire No.1.Under high temperature stress for 1-36 hours,expression level of MiZFP11 in leaves increased with"up-down"patterns in JW and Guire No.1,and expression level in JW was significantly higher than that of Guire No.1.Therefore,MiZFP11 was significantly expressed in different tissues of macadamia,and was significantly up-regulated under drought and high temperature stresses,which speculated that MiZFP11 gene plays an important role in the growth and development of macadamia tissues and the response to drought and high temperature stresses.关键词
澳洲坚果/CCCH型锌指蛋白/干旱胁迫/高温胁迫/功能分析Key words
macadamia/CCCH zinc finger protein/drought stress/high temperature stress/functional analysis引用本文复制引用
杨祥燕,蔡元保,曾黎明,杨为海,林玉虹,巫辅民,赵渊,胡玲..澳洲坚果锌指蛋白基因MiZFP11响应干旱和高温胁迫的功能分析[J].热带作物学报,2025,46(3):524-533,10.基金项目
国家自然科学基金项目(No.32060652) （No.32060652）
广西科技计划项目(桂农科AB241484005) （桂农科AB241484005）
广西农业科学院基本科研业务专项(桂农科2021YT154). （桂农科2021YT154）
