热带作物学报2025，Vol.46Issue(3)：534-543,10.DOI:10.3969/j.issn.1000-2561.2025.03.003
巴西橡胶树HbbHLH48基因克隆与亚细胞定位分析
Cloning and Subcellular Localization Analysis of HbbHLH48 Gene from Hevea brasiliensis Muell.Arg.
摘要
Abstract
Plant bHLH transcription factor is one of the key factor of ethylene and jasmonic acid hormone signal trans-duction pathway,and plays an important role in the yield formation and latex flow in rubber trees.A bHLH transcription factor was cloned from the leaves of Reyan73397 designed as HbbHLH48(GenBank:PQ303617),with a total length of 1128 bp encoding 375 amino acids.Its encoded protein has the highest similarity to Arabidopsis AtbHLH48,and be-longs to the XVII subgroup.Bioinformatics analysis showed that it contained the bHLH_SF superfamily domain,but did not contain the transmembrane domain and signal peptide.The expression levels in flowers,leaves and latex were high-er than those in roots and stems.Mechanical injury treatment down-regulated its expression in tapping trees.Methyl jasmonate,salicylic acid,and ethephon treatments initially significantly upregulate their expression and then downregulate it,while the expression pattern with gibberellin treatment is opposite to them.Subcellular localization analysis revealed it to be in the nucleus.It can be seen that HbbHLH48 is a member of the plant bHLH transcription factor family,and its function in Hevea brasiliensis may be involved in the gibberellin process,thereby regulating the flowering process and latex biosynthesis process,laying the foundation for further in-depth research.关键词
巴西橡胶树/HbbHLH48/克隆/表达分析/亚细胞定位Key words
Hevea brasiliensis Muell.Arg./HbbHLH48/clone/expression analysis/subcellular localization分类
林学引用本文复制引用
范晓康,樊松乐,刘明洋,郭冰冰,杨洪,代龙军,张宇,王立丰..巴西橡胶树HbbHLH48基因克隆与亚细胞定位分析[J].热带作物学报,2025,46(3):534-543,10.基金项目
海南省自然科学基金创新研究团队项目(No.322CXTD506) （No.322CXTD506）
海南省自然科学基金高层次人才项目(No.323RC526) （No.323RC526）
热带作物病虫害疫情监测与防控项目(No.21240116). （No.21240116）
