热带作物学报2025，Vol.46Issue(3)：544-552,9.DOI:10.3969/j.issn.1000-2561.2025.03.004
文心兰软腐病菌Dickeya fangzhongdai Onc5 vfmE基因的克隆及表达分析
Cloning and Expression Analysis of vfmE gene from Soft Rot Patho-gen Dickeya fangzhongdai Onc5 of Oncidium
摘要
Abstract
Dickeya fangzhongdai is one of the main pathogens causing the devastating disease(soft rot disease)of Or-chidaceae.Pathogenic genes play a crucial role in the infection process.Dickeya fangzhongdai Onc5 was isolated from the diseased plant(Oncidium,Gower Ramsey)in Fujian province.Pathogenic gene play an important role in the inter-action between pathogens and plants.The purpose of this study was to larify the molecular biological characteristics of D.fangzhongdai Onc5 vfmE gene and analysis its role in the infection process,so as to provide theretical basis for fur-ther reveal the pathogenic mechanism of D.fangzhongdai Onc5 vfmE gene in the infection process.Using the D.fang-zhongdai Onc5 DNA as template,of vfmE gene was amplified by reverse transcription-polymerase chain reaction(RT-PCR)and its bioinformatics were analyzed.The expression of vfmE gene was analyzed during D.fangzhongdai Onc5 infecting Oncidium by quantitative reverse transcription PCR(RT-qPCR).The result showed that the ORF se-quence of vfmE gene was 564 bp,encoding 187 amino acids with a molecular weight of 21.93 kDa and isoelectric point of 9.01 and a conserved AraC-superfamily domain.The vfmE protein was an unstable hydrophilic protein without signal peptide and transmembrane structure.The secondary structure of protein was mainly composed of α-helix(58.29%)and random coil(40.64%).Sequence similarity analysis revealed that the translated molecules showed high homology with other pathogens in the genus Dickeya(>86%).RT-qPCR analysis showed that vfmE gene was highly expressed con-tinuously throughout the infection process of D.fangzhongdai Onc5,and the highest expression level was at 12 h.vfmE protein plays an important role in the infection of D.fangzhongdai Onc5.Research on the pathogeneisis-related gene vfmE in D.fangzhongdai Onc5 provide a theoretical basis for further research on the vfmE gene function and pathogenic regulation mechanism in Dickeya genus.Furthermore,such studies may be useful for finding new target to control plant soft rot disease.关键词
狄克氏菌/vfmE基因/克隆/生物信息学分析/表达分析Key words
Dickeya fangzhongdai Onc5/vfmE gene/cloning/bioinformatics analysis/expression analysis分类
植物保护学引用本文复制引用
王文婷,任驰,丁建华,荆恩荣,卢秋巍..文心兰软腐病菌Dickeya fangzhongdai Onc5 vfmE基因的克隆及表达分析[J].热带作物学报,2025,46(3):544-552,9.基金项目
山西省科技厅基础研究计划项目(No.20210302124516,No.20210302124502) （No.20210302124516,No.20210302124502）
山西省高校科技创新计划项目(No.2021L462). （No.2021L462）
