热带作物学报2025，Vol.46Issue(3)：553-562,10.DOI:10.3969/j.issn.1000-2561.2025.03.005
莲雾调控开花基因SsSOC1的克隆及表达分析
Cloning and Expression Analysis of the Flowering Gene SsSOC1 in Wax Apple
摘要
Abstract
SOC1(SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1)is a flowering time regulator in plants.It belongs to the MADS-box transcription factor family and plays a crucial role in the transition from vegetative to repro-ductive growth in plants.SOC1 is involved in various biological processes,including flower organ development,flow-ering time control,and response to environmental stimuli and hormone signals.In this study,shading and application of Lorsben were used for flower induction in wax apple,and the number of leaf buds,flowers,and the rate of flowering shoots were recorded after treatment.Additionally,the existing transcriptome data of wax apple in our research group were analyzed to identify the candidate gene SsSOC1 involved in regulating flowering in wax apple.The differential expression of SsSOC1 in different stages and tissues of wax apple under shading and Lorsben treatment was studied using qPCR.The results showed that the expression of the SsSOC1 gene significantly increased in the buds,leaves,leaf tips,and petioles of wax apple treated with shading,with the highest relative expression in the leaf tips.In this study,the SsSOC1 gene from Hei Tang Ba Bi wax apple was successfully cloned,and its promoter analysis,physicochemical properties of the protein,prediction of conserved functional domains,and sequence alignment were performed using bioinformatics methods.The open reading frame of the SsSOC1 gene in wax apple was found to be 654 bp,encoding a protein of 217 amino acids.The theoretical isoelectric point of the protein was 9.37,with a molecular formula of C1076H1794N322O334S10 and a molecular weight of 24.91 kDa.The protein contained 40 positively charged amino acid residues(Lys+Arg)and 32 negatively charged amino acid residues(Asp+Glu).The protein had a fat coefficient of 80.64,a grand average of hydropathicity(GRAVY)of –0.729,and an instability coefficient of 66.17.The protein structure lacked β-folds and did not belong to secretory or membrane proteins.Subcellular localization analysis indicated that the protein was mainly present in the nucleus and belonged to an unstable hydrophilic basic protein.The SsSOC1 protein in wax apple possessed conserved domains including MADS_MEF2_like and K-Box and belonged to the MADS super-family and K-Box superfamily.The analysis identified the amino acid positions 3 to 75 and 88 to 171 as the start and end sites of the conserved domains,respectively,conforming to the typical structural features of the MADS protein family and the K-Box family.The evolutionary tree showed the highest homology between the SsSOC1 sequence in wax apple and Syzygium oleosum.By floral dip transformation,the SsSOC1 gene was introduced into Arabidopsis,and it was found that the 35S::SsSOC1 line exhibited an early flowering phenotype and a reduced number of rosette leaves com-pared to the wild type.This study preliminarily elucidated the important role of the SsSOC1 gene in flowering in wax apple and attempted to reveal the intrinsic regulatory mechanism of shading-induced flowering,providing guidance for wax apple production and theoretical basis for improving out-of-season cultivation techniques.关键词
莲雾/成花/SsSOC1/基因克隆/转录活性分析/表达模式分析Key words
wax apple/flowering/SsSOC1/gene cloning/transcriptional activity analysis/expression pattern analysis分类
植物保护学引用本文复制引用
杨胤延,孙东宇,蔡昕成,胡志群,吴国麟,周碧燕..莲雾调控开花基因SsSOC1的克隆及表达分析[J].热带作物学报,2025,46(3):553-562,10.基金项目
国家自然科学基金项目(No.32072515) （No.32072515）
广州市重点研发计划项目(No.202206010023). （No.202206010023）
