热带作物学报2025，Vol.46Issue(3)：563-572,10.DOI:10.3969/j.issn.1000-2561.2025.03.006
番茄OSCA家族成员的敲除突变体构建及抗旱性研究
Establishment of Knockout Mutants for OSCA Family Members and Drought Tolerance Research in Tomato
摘要
Abstract
Drought is one of the most serious abiotic stresses to land plants.Environmental water deficiency triggers an osmotic stress signaling cascade.OSCA1 was described as a hyperosmolarity-gated calcium-permeable channel and its function in osmosensing in plants,homologues of OSCA genes are found throughout eukaryotes.Water is crucial to plant growth and development.As one of the primary horticulture commodities,tomato plays an important role in vegetable research.To enhance the survival and yield of tomatoes in drought conditions,it is essential to thoroughly study the function of SlOSCA genes and their drought-resistant mechanisms.Based on reverse genetics,we selected SlOSCA1.1/1.2/1.6/2.3/3.1 as our target genes through bioinformatics analysis,the slosca mutant of tomato was made by CRISPR/cas9-mediated gene editing directly in AC(Ailsa Craig).To explore the role of tomato SlOSCA protein family members in the response to osmotic stress.Here,we found impaired guard cell osmotic stress signaling and attenuated plant responses to osmotic stress in slosca3.1/1.1 plants,impaired osmotic stress signaling and attenuated plant re-sponses to osmotic stress in slosca1.6/1.2/2.3 plants,the slosca1.6/1.2/2.3 plants showed lower water use efficiency and poorer drought resistance,there is genetic redundancy in OSCA.In this study,slosca mutants were constructed and then the physiological phenotypes of the mutants were investigated,which revealed that multiple genes of the SlOSCA fam-ily cooperatively regulate responses to osmotic stress,this study provides a reference for subsequent analysis of the biological functions of OSCA gene family under different stress conditions and different physiological processes.关键词
OSCA基因家族/干旱胁迫/基因编辑/番茄Key words
OSCA family/drought stress/gene editing/tomato分类
园艺学与植物营养学引用本文复制引用
王菲,王焱,杨伟光,代世文,熊程,邹学校,裴真明,裴宋雨,远方..番茄OSCA家族成员的敲除突变体构建及抗旱性研究[J].热带作物学报,2025,46(3):563-572,10.基金项目
国家"十四五"重点研发计划项目(No.2023YFF1001200). （No.2023YFF1001200）
