热带作物学报2025，Vol.46Issue(3)：573-584,12.DOI:10.3969/j.issn.1000-2561.2025.03.007
桉蝙蛾嗅觉相关蛋白的鉴定及部分新鉴定蛋白的表达分析
Identification of Olfactory Related Proteins and Expression Analysis of Some Newly Identified Proteins in Endoclita vietnamensis
摘要
Abstract
Endoclita vietnamensis Buchsbaum&Grehan,a newly discovered pest of dried Eucalyptus,is a typical ex-ample of native insect adaptation to an alien host.Adult larvae scatter the eggs on the ground below the forest,and the larvae live in the litter layer to the third stage after hatching,and then transfer the damage to the tree.Even in mixed forests,larvae can accurately locate the host,which leads to be assumed that the olfactory system of larvae is conducive to host selection.In addition,the olfactory system also plays an important role in the mating process of adult worms.Here,we studied the transcriptome of the head,chest and abdomen of the 5th instar,12th instar and antennal antennae of the adult,and investigated the expression profile of olfactory proteins.A total of 66 olfactory proteins were identified,14 of which were newly identified,including three odor-binding proteins(OBPs),four odor receptors(ORs),one taste receptor(GRs),and six ionic receptors(IRs).The expression profiles showed that all OBPs,ORs and GRs were ex-pressed to varying degrees in the three stages,except OR22 and OR25 which were not expressed in the head of the 5th instar larvae.The expression level of EsigOR23 was the highest among all olfactory proteins,and was significantly high in the head of 5th instar larvae.The expression of EsigGOBP9 in adult antennae was significantly higher than that in adults at 5 and 12 ages.This study would provide a molecular basis for further study of the chemical sensation of the E.vietnamensis.EsigOR23 and EsigGOBP9 have unique expression patterns,which may be important proteins affecting host selection in larvae and courtship behavior in adults,respectively,and deserve further exploration.关键词
桉蝙蛾/表达谱/嗅觉相关蛋白/转录组Key words
Endoclita vietnamensis/expression profile/olfactory associated protein/transcriptome分类
生物学引用本文复制引用
陈宇,胡平..桉蝙蛾嗅觉相关蛋白的鉴定及部分新鉴定蛋白的表达分析[J].热带作物学报,2025,46(3):573-584,12.基金项目
广西壮族自治区科技基地和人才专项(No.2020AC19057) （No.2020AC19057）
国家自然科学基金青年科学基金项目(No.32001321) （No.32001321）
广西壮族自治区自然科学基金面上项目(No.2020JJA130068). （No.2020JJA130068）
