热带作物学报2025，Vol.46Issue(3)：611-628,18.DOI:10.3969/j.issn.1000-2561.2025.03.010
超低温保存技术在热带作物种质资源保存中的应用与展望
Applications and Prospects of Cryogenic Technology in the Conserva-tion of Tropical Crop Germplasm Resources
摘要
Abstract
Tropical region is rich in plant genetic diversity with great demand for the safe preservation of plant germ-plasm resources.Cryopreservation[with liquid nitrogen(LN)]is an important method for the long-term preservation of plant germplasm resources.Although cryopreservation protocol often requires optimization for different species,it en-sures the use of minimum space and the regular LN supply to facilitate the long-term preservation of genetic resources once established,thereby greatly reducing the labor input and costs in the long run.The cryogenic technique has been studied for more than half a century and has achieved survival and regeneration of plant materials such as seeds,shoot tips and pollen,as well as cell suspension and callus tissue after cryopreservation.To fully introduce the use of cryo-preservation to secure valuable tropical plant species and support the development of tropical agricultural sciences,this review first focuses on the shoot tip and dormant shoot segment cryopreservation to review the research history and technological development of plant cryopreservation.The research advances of cryopreservation in tropical plant spe-cies were emphasized using shoot tips,seeds(zygotic embryos),pollen,cell suspension,and callus.The importance of genetic stability analysis after cryopreservation was also introduced before highlighting the importance and prospects of applying cryopreservation for long-term preservation of shoot tips,recalcitrant seeds,and embryogenic tissues and cells of tropical crops.关键词
热带作物/超低温保存/种质资源/遗传稳定性Key words
tropical crops/cryopreservation/germplasm resources/genetic integrity分类
植物保护学引用本文复制引用
王敏瑞,荆永琳,陈浪欣,王小冰,孟春阳,黄碧兰,徐立,李志英..超低温保存技术在热带作物种质资源保存中的应用与展望[J].热带作物学报,2025,46(3):611-628,18.基金项目
国家重点研发计划项目(No.2021YFC2600603) （No.2021YFC2600603）
中央级公益性科研院所基本科研业务费专项(No.1630032023011). （No.1630032023011）
