热带作物学报2025，Vol.46Issue(3)：705-715,11.DOI:10.3969/j.issn.1000-2561.2025.03.018
不同提取方法对番石榴叶有效成分、抗氧化及α-糖苷酶抑制活性的影响
Effects of Extraction Methods on Effective Constituents,Antioxidant Activities,α-glycosidase Inhibition of Guava Leaves
摘要
Abstract
Guava leaf(Psidium guajava Linn.)is a common folk medicine in China.Modern pharmacological studies have confirmed its remarkable antioxidant and hypoglycemic activities,which has broad prospects in the development of natural antioxidants and hypoglycemic agents.The release of non-volatile active components of plants or herbs were greatly affected by extraction ways.The extraction effects on guava leaves of macerated extraction(ME),heat reflux extraction(HRE),ultrasound-assisted alcohol extraction(UAAE)and ultrasound-assisted deep eutectic solvents extrac-tion(UADEE),microstructure were evaluated.The content of total saponins,total flavonoids,total phenols in the ex-tract was respectively determined by vanillin-glacial acetic acid colorimetric method,NaNO2-Al(NO3)3 colorimetric method and folin phenol method.The content of flavonoid components was determined by high performance liquid chromatography(HPLC).The metabolites were analyzed by liquid chromatograph-mass spectrometer(LC-MS).In vitro antioxidant activity and α-glycosidase inhibitory ability of the extract were also evaluated.In UADEE group,internal structure of guava leaves was most completely damaged,the content of total saponins,total flavonoids,total phenols and guaijaverin,avicularin,reynoutrin was respectively determined to be 79.28 mg/g,80.51 mg/g,120.52 mg/g,7.96 mg/g,7.74 mg/g and 3.99 mg/g,which significantly higher than those of the other three groups(P<0.05).Flavon-oids,triterpenoids and phenols accounted for 9.830%,27.267%and 1.013%of all metabolites in the extract,respectively.The IC50 value of scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals and inhibiting α-glycosidase in UADEE group was 45.18 μg/mL and 904.67 μg/mL,respectively,significantly superior than those of the other three groups(P<0.05).In summary,UADEE has the best effect on the extraction of active components in the extract,attributed to the mechanical and chemical effects of ultrasound and the polarity tunability and solubilization of solvents.The results would provide experimental basis for the efficient extraction of effective components from guava leaves and the devel-opment of processed products.关键词
番石榴叶/超声辅助低共熔溶剂法/有效成分/DPPH自由基清除率/α-糖苷酶抑制活性Key words
guava leaves/ultrasound-assisted deep eutectic solvents extraction/active constituents/1,1-diphenyl-2-pic-rylhydrazyl radical scavenging ability/α-glycosidase inhibitory ability分类
农业科技引用本文复制引用
罗朝丹,王秋菊,李建强,冯春梅,任二芳,黄燕婷,宁琳,罗小杰..不同提取方法对番石榴叶有效成分、抗氧化及α-糖苷酶抑制活性的影响[J].热带作物学报,2025,46(3):705-715,11.基金项目
广西农业科学院科技发展基金项目(桂农科2022JM92) （桂农科2022JM92）
广西农业科学院基本科研业务专项(桂农科2025YP140,桂农科2021YT144). （桂农科2025YP140,桂农科2021YT144）
