热带作物学报2025，Vol.46Issue(3)：734-742,9.DOI:10.3969/j.issn.1000-2561.2025.03.021
高产IAA抗旱菌株SZF7的培养条件优化及功能基因挖掘
Optimization of Culture Conditions and Functional Gene Mining of High-yielding IAA Drought-resistant Strain SZF7
摘要
Abstract
Drought resistant microbial preparations are beneficial soil microorganisms that produce plant growth hor-mone to enhance the drought tolerance of plants and promote plant growth,which is an ideal substitute for traditional chemical drought resistant preparations.In this study,the bacteria obtained in the soils of Wuyishan tea plantation were used as the research object to screen strains with the high secretion indole-3-acetic acid(IAA)ability and strong drought resistance,and the culture conditions optimization and whole genome sequencing analysis were also conducted.A strain SZF7 with high secreting IAA was obtained,which belonged to Bacillus cereus,which had stronger drought resistance than other strains.The optimal culture conditions for IAA secretion of SZF7 were pH 5.0 and 37℃with 24 h incuba-tion,and pH and temperature significantly affected on the IAA secretion.In the COG and KEGG metabolic pathways of SZF7 genome,the genes related to biological metabolism accounted for 47.96%and 68.52%of the total functional genes,respectively.The genes related to plant growth promotion included IAA,siderophore,non-ribosomal peptide synthase(NRPS),lipopeptide compounds and bacillibactin.There were two IAA synthesis pathways in SZF7,one of which was indole-3-pyruvate pathway mediated by indole-3-pyruvate decarboxylase,and the other was tryptamine pathway mediated by indole-3-glycerol phosphate synthase.This study would provide a scientific basis for the applica-tion of drought-resistant and growth-promoting bacteria in the production of tea and other cash crops.关键词
促生菌/吲哚乙酸/抗旱/培养条件优化/全基因组分析Key words
growth-promoting bacteria/indole-3-acetic acid/drought resistance/optimization of culture conditions/whole genome analysis分类
农业科技引用本文复制引用
林宏,黄玉莲,黄卫红,李若雨,薛喜枚,张英娇,张秋芳..高产IAA抗旱菌株SZF7的培养条件优化及功能基因挖掘[J].热带作物学报,2025,46(3):734-742,9.基金项目
福建省科技厅引导性项目(No.2020N0032,No.2022N0026) （No.2020N0032,No.2022N0026）
国家重点研发计划项目(No.2023YFD1701703). （No.2023YFD1701703）
