日用化学工业（中英文）2025，Vol.55Issue(3)：399-406,8.DOI:10.3969/j.issn.2097-2806.2025.03.016
UHPLC-MS/MS法检测化妆品中21种非甾体抗炎药
Determination of 21 nonsteroidal anti-inflammatory drugs in cosmetics by high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was developed for the determination of 21 nonsteroidal anti-inflammatory drugs(NSAIDs)in cosmetics by ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Four kinds of cosmetics such as cream,facial mask,gel and toner were selected as representative matrix.The sample pretreatment conditions,chromatography and mass spectrometry conditions were systematically investigated.At first,the cosmetic samples were extracted by methanol for cream,gel,toner matrix,and methanol containing 0.3%(V/V)formic acid for facial mask matrix by ultrasonic-assisted liquid-liquid extraction.Then they were separated on an ACQUITY UPLC BEH C18 column(1.7 μm,2.1 mm×150 mm)by gradient elution.Aqueous containing 0.1%(V/V)formic acid and acetonitrile were used as mobile phases.The flow rate was 0.3 mL/min and injection volume was 5 μL.Finally,samples were detected with multiple reaction monitoring(MRM)scanning mode.The results show a good linearity in the concentration range of 10-100 μg/L for rofecoxib and 5-50 μg/L for other NSAIDs.The spiked recoveries at the low,medium,and high levels range from 72.1%to 117.3%,with the RSD ranging from 0.6%to 9.7%(n=6).This method has the characteristics of simple operation,high accuracy,and good sensitivity.It is suitable for the detection of NSAIDs in cosmetics,and provides a technical support for the supervision in cosmetics.关键词
超高效液相色谱-串联质谱/化妆品/非甾体抗炎药Key words
ultra-high performance liquid chromatography-tandem mass spectrometry/cosmetics/nonsteroidal anti-inflammatory drugs(NSAIDs)分类
化学化工引用本文复制引用
冯克然,吴晓鸣,马亮波,孙宇..UHPLC-MS/MS法检测化妆品中21种非甾体抗炎药[J].日用化学工业（中英文）,2025,55(3):399-406,8.基金项目
科技部重点研发计划项目《一站式检验检测服务协同平台示范应用》(2018YFB1403305) （2018YFB1403305）
