山西农业科学2025，Vol.53Issue(2)：18-25,8.DOI:10.3969/j.issn.1002-2481.2025.02.04
麦类作物功能活性多肽Lunasin研究进展
Research Progress of Functional Bioactive Peptide Lunasin in Triticeae Crop
摘要
Abstract
Lunasin,a small molecule bioactive peptide,was initially isolated from soybean.Lunasin peptide exhibited multiple activities based on its specially structure,which consists of arginine-glycine-aspartate cell adhesion motif(RGD),α-helix structure,and nine C-terminal aspartate residues(Poly-D).At present,lunasin production mainly includes three approaches of isolation from natural raw materials,solid-phase synthesis according to amino acid sequence,and biosynthesis via organism's expression system.Although Lunasin has great application potential,its content in natural materials such as wheat is low,resulting into high cost in extraction.It is particularly important to explore the molecular mechanism of Lunasin synthesis,and to improve the production efficiency.In this review,integrating recent research progresses,the contents of Lunasin and its structurally similar peptides in the triticeae crops such as wheat,barley,and oat were summarized.Furthermore,the technology of Lunasin extraction,separation,purification,and identification by mass spectrometry were listed.Besides,the physiological functions of Lunasin including antioxidant,anti-inflammatory,anticancer,cholesterol-lowering,and anti-obesity were summarized in detail based on its specific structure characteristics.Finally,the methods of Lunasin biosynthesis were summarized such as prokaryotic expression in Escherichia coli chassis and eukaryotic expression in yeast chassis.And the research direction including the molecular mechanism associated with Lunasin synthesis in wheat and the identification of QTL sites were prospected.关键词
Lunasin/生物活性/分离纯化/生产合成/基因工程/遗传基础Key words
Lunasin/biological activity/separation and purification/production and synthesis/genetic engineering/genetic basis分类
农业科技引用本文复制引用
郝宇琼,任贵兴..麦类作物功能活性多肽Lunasin研究进展[J].山西农业科学,2025,53(2):18-25,8.基金项目
山西省基础研究计划自由探索类面上基金(202303021221098) （202303021221098）
山西农业大学高层次人才启动项目(2023BQ110) （2023BQ110）
