山西农业科学2025，Vol.53Issue(2)：67-74,8.DOI:10.3969/j.issn.1002-2481.2025.02.09
抗白粉病小偃麦1St异附加系的分子细胞遗传学鉴定
Molecular Cytogenetic Identification of Wheat-Thinopyrum ponticum 1St Disomic Addition Line with Powdery Mildew Resistance
摘要
Abstract
Wheat powdery mildew,causing serious yield loss of wheat,is one of the major diseases in wheat-planting areas around the world.Mining new disease-resistant genes,creating and utilizing new disease-resistant germplasms are considered to be the most effective ways to improve the disease resistance of wheat.Thinopyrum ponticum carries many useful genes and serves as a valuable gene bank for wheat genetic improvement.In the present study,new Trititrigia germplasm SN21147 was developed by crossing wheat-Th.ponticum octoploid SNTE20 with common wheat.Disease evaluation showed that SN21147 was immune and high resistant to powdery mildew at the seedling and adult stage,respectively.Sequential GISH-FISH found that SN21147 had 42 wheat chromosomes plus a pair of Th.ponticum chromosomes.Furthermore,chromosome structural variations were also detected on the wheat chromosomes 6A,7A,7B,1D,2D,and 7D.Using IT(Intron Targeting)marker,twelve exogenous specific markers were obtained,six of which belonged to the St genome and homoeologous group one of Pseudoroegneria spicata.These results suggested that SN21147 was 1St disomic alien addition line.Besides,SN21147 had a lower plant height(56.7 cm),more tillers and grains per spike compared to its common wheat parent SN637,the two traits increased by 44.4％ and 4.3％ .Therefore,SN21147 could be utilized as a new germplasm with powdery mildew resistance in wheat genetic improvement.关键词
长穗偃麦草/白粉病/原位杂交/分子标记Key words
Thinopyrum ponticum/powdery mildew/in situ hybridization/molecular marker分类
农业科技引用本文复制引用
刘宝磊,房方,杨国堂,李兴锋,于海涛,鲍印广..抗白粉病小偃麦1St异附加系的分子细胞遗传学鉴定[J].山西农业科学,2025,53(2):67-74,8.基金项目
山东省重点研发计划(重大科技创新工程)(2021LZGC009) （重大科技创新工程）
