生态学杂志2025，Vol.44Issue(3)：848-856,9.DOI:10.13292/j.1000-4890.202503.047
喀斯特峡谷石漠化区造林对土壤细菌群落结构的影响
Influence of afforestation on soil bacterial community structure in karst canyon rocky desertification area
摘要
Abstract
Soil microorganisms are important indicator for evaluating the effectiveness of vegetation restoration in karst ecosystems.However,the response of soil microbial communities to vegetation restoration remains uncertain due to the influence of biotic and abiotic factors.Whether soil physicochemical properties,as one of the main fac-tors affecting the habitat of bacteria,can be directly used to infer variations in soil bacterial communities associated with land use changes in karst ecosystems remains unclear.Here we collected soil samples of surface layer(0-10 cm depth)from Zanthoxylum bungeanum forest and secondary forest in the rocky desertification area of Huajiang Karst Canyon,Guizhou,as well as cropland as a control.By using high-throughput 16S rRNA gene sequencing technology,we examined the effects of afforestation on soil properties,soil bacterial community composition,and diversity in karst rocky desertification area,as well as the main factors affecting soil bacterial community after affor-estation.The results showed that the main dominant phyla of soil bacteria in all samples were Acidobacteriota,Acti-nobacteriota,Proteobacteria,and Chloroflexi.Afforestation significantly altered the abundance but not alpha diver-sity of soil bacteria.After cropland was converted to Zanthoxylum bungeanum forest and secondary forest,the rela-tive abundance of Acidobacteriota and Actinobacteriota significantly increased,while that of Proteobacteria signifi-cantly decreased.Chloroflexi had the highest abundance in Zanthoxylum bungeanum forest.Afforestation reduced the abundance of significantly enriched bacteria and changed the type of enriched bacteria.Redundancy analysis showed that soil pH,exchangeable calcium,organic carbon,water content,bulk density,and soil silt content jointly affected bacterial community structure,with exchangeable calcium having the highest degree of influence.These results indicated that the potential interaction between soil physicochemical properties and bacterial communi-ties may jointly affect soil bacterial community structure after afforestation in karst canyon areas.关键词
造林/交换态钙/土壤细菌群落/喀斯特石漠化Key words
afforestation/exchangeable Ca/soil bacterial community/karst rocky desertification引用本文复制引用
王莎莎,蓝家程,祁雪,王俊贤,龙启霞,黄明芝,刘磊,岳坤前..喀斯特峡谷石漠化区造林对土壤细菌群落结构的影响[J].生态学杂志,2025,44(3):848-856,9.基金项目
国家自然科学基金(42177446和41601584)、贵州省科学技术基金(黔科合[2017]1417)和贵州师范大学(黔师新苗[2022]28)资助. （42177446和41601584）
