护理研究2025，Vol.39Issue(6)：947-953,7.DOI:10.12102/j.issn.1009-6493.2025.06.010
金丝桃苷降低小鼠血浆胆固醇水平的效果研究
Study on the effect of hypericin in reducing plasma cholesterol levels in mice
摘要
Abstract
Objective:To intestigate the ability and mechanism of action of hyperoside in reducing plasma total cholesterol levels through in vitro and in vivo experiments.Methods:The mice model with hypercholesterolemia was established by feeding them high-fat and high-cholesterol chow,the hypericin intervention group and positive control group received different doses of hyperoside and lovastatin by gavage for 8 weeks.The changes in weight of the mice in each group were recorded.The content of serum cholesterol related indicators in mice were measured.The pathological changes and lipid accumulation of hepatic tissues and the uptake capacity of the hepatocytes for low-density lipoprotein cholesterol(LDL-C)was observed.The abundance of low density lipoprotein receptor(LDLR)proteins in the hepatocytes and hepatic tissues was detected after the intervention of hyperoside.Results:The mice model of hypercholesterolemia was successfully established.Compared to the hypercholesterolemia model group,the mice of hypericin intervention group showed a significant decrease in blood total cholesterol(TC)levels(P<0.05),and low-density hypericin intervention group showed a significant decrease in LDL-C(P<0.05).The hepatic tissues of mice in the hypercholesterolemia model group exhibited a large number of vacuolated steatosis,and the hepatocytes were sparse and disorganized.The hepatic tissues steatosis and the number of lipid droplets was significantly reduced in the hypericin intervention group compared to thehypercholesterolemia model group.The vitro experiments showed that hyperoside significantly increased TC levels and LDL-C content in hepatocytes(P<0.01),promoted LDL-C uptake in hepatocytes and increased abundance of LDLR in hepatocytes and hepatic tissues of mice(P<0.01).Conclusions:Hyperoside may promote the uptake of LDL-C in the hepatic tissues of mice by increasing the abundance of LDLR,thereby reducing the plasma total cholesterol levels of mice.关键词
金丝桃苷/高胆固醇血症/食药两用植物(EMPs)/总胆固醇/低密度脂蛋白胆固醇/低密度脂蛋白受体Key words
hypericin/hypercholesterolemia/edible and medicinal plants,EMPs/total cholesterol/low-density lipoprotein cholesterol/low density lipoprotein receptor引用本文复制引用
仝彤,孙嘉颖,史艳楠,邵莹,杨利军..金丝桃苷降低小鼠血浆胆固醇水平的效果研究[J].护理研究,2025,39(6):947-953,7.基金项目
山西省基础研究计划青年项目,编号:20210302124415 （）
山西省基础研究计划面上项目,编号:202203021211229 （）
山西省科技创新人才团队专项计划项目,编号:202204051002030 （）
