实验科学与技术2025，Vol.23Issue(2)：128-132,5.DOI:10.12179/1672-4550.20230169
BD FACSAria流式分选仪无菌制备流程的优化
Optimization of the Aseptic Preparation Process for BD FACSAria Flow Sorter
杨欢 1郭英华 1张雪芳 1罗佳 1杜立颖 1吕红霞1
作者信息
- 1. 北京大学生命科学学院,北京 100871||国家蛋白质研究中心(北京)北京大学分中心,北京 100871
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摘要
Abstract
To optimize the process of aseptic preparation and maintenance for BD FACSAria cell sorter to maintain long-term stable sterile sorting state,the cost of aseptic preparation,operation process and system stability are optimized.The method of monitoring the aseptic status of the fluidics system with LB medium is explored,and the aseptic sorting status and long-term culture of HCT116 cells are observed.The optimized aseptic preparation methods simplify the operation process,save the cost,and can keep the aseptic state of the instrument liquid flow pipeline for a long time.HCT116 colorectal cancer cells are sorted and cultured in antibiotic-free medium for a long time without contamination.The optimized aseptic preparation methods and maintenance procedure of BD FACSAria sorter provide a simple and feasible scheme for the operation and management of the instrument,and provide high-quality and stable aseptic sorting services for scientific research.关键词
流式分选仪/无菌制备优化/无菌维护/无菌分选Key words
flow cytometry sorter/optimization of aseptic preparation/aseptic maintenance/aseptic sorting分类
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杨欢,郭英华,张雪芳,罗佳,杜立颖,吕红霞..BD FACSAria流式分选仪无菌制备流程的优化[J].实验科学与技术,2025,23(2):128-132,5.
